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Prothrombin Antibody (9A1)

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Immunohistochemistry: Prothrombin Antibody (9A1) [NBP3-26594] - Image of Prothrombin Antibody (9A1) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC
Clone
9A1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Prothrombin Antibody (9A1) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human Prothrombin [UniProt P00734]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
F2
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Prothrombin Antibody (9A1)

  • coagulation factor II (thrombin)
  • Coagulation factor II
  • EC 3.4.21
  • EC 3.4.21.5
  • prothrombin B-chain
  • prothrombin
  • PT
  • serine protease

Background

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Prothrombin Antibody (NBP3-26594)

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Blogs on Prothrombin.

Factor VIII - a key factor in the clotting process
Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation...  Read full blog post.

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Bioinformatics

Gene Symbol F2