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Protein phosphatase 1F Antibody

Images

 
Independent Antibodies: Western Blot: Protein phosphatase 1F Antibody [NBP1-88207] - Analysis using Anti-PPM1F antibody NBP1-88207 (A) shows similar pattern to independent antibody NBP1-88206 (B).
Immunocytochemistry/ Immunofluorescence: Protein phosphatase 1F Antibody [NBP1-88207] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Protein phosphatase 1F Antibody [NBP1-88207] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in glomeruli.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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Protein phosphatase 1F Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LDTLLQDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRKAPPPLAAALAHEAVSQLLQTDLSEFRKLPRE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPM1F
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Protein phosphatase 1F Protein (NBP1-88207PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Protein phosphatase 1F Antibody

  • Ca(2+)/calmodulin-dependent protein kinase phosphatase
  • CaMKPaseCaM-kinase phosphatase
  • EC 3.1.3.16
  • FEM-2
  • hFEM-2
  • KIAA0015POPX2CAMKP
  • Partner of PIX 2
  • PP2C phosphatase
  • Protein fem-2 homolog
  • protein phosphatase 1F (PP2C domain containing)
  • protein phosphatase 1F
  • protein phosphatase, Mg2+/Mn2+ dependent, 1F

Background

Protein phosphatase 1F is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPM1F