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Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

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ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-80325] - Example standard curve for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) [NBP2-80325] - Example standard curve for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric). These standard curves are provided for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP
Suitable Sample Type
Lysate
Standard Curve Range
125-8000 pg/ml (example only; lot dependent)
Sensitivity
42.02 pg/ml

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Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric) Summary

Specificity
Recognizes both recombinant and natural Human Protein Disulfide Isomerase/P4HB.
Standard Curve Range
125-8000 pg/ml (example only; lot dependent)
Sensitivity
42.02 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 5.3 %
Intra-Assay
CV% < 3.2
Spike Recovery
78-82%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
P4HB

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. 1. 96 well microplate coated with Capture Antibody
  2. 2. Detection Antibody conjugated to HRP
  3. 3. Standards
  4. 4. Wash Buffer Concentrate
  5. 5. Dilution Buffer Concentrate
  6. 6. Color Reagent A
  7. 7. Color Reagent B
  8. 8. Stop Solution

Alternate Names for Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Manual - Human Protein Disulfide Isomerase/P4HB ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol P4HB