![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3225) labeled with CF488 (Green); Reddot is used to label the nuclei.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-PHB-3231-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79879-img0003.jpg "Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3225) labeled with CF488 (Green); Reddot is used to label the nuclei.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, MA |
| Clone | PHB/3231 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79744). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Prohibitin protein fragment (aa167-261) (exact sequence is proprietary) (Uniprot: P35232) |
| Localization | Cytoplasmic (Mitochondria) |
| Marker | Mitochondrial Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PHB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Prohibitin Antibody (NBP2-79879)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PHB1 |
![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3231).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-PHB-3231-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79879-img0002.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3231).")
![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3231).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-PHB-3231-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79879-img0001.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3231).")
![Protein Array: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-PHB-3231-Azide-and-BSA-Free-Protein-Array-NBP2-79879-img0004.jpg "Protein Array: Prohibitin Antibody (PHB/3231) - Azide and BSA Free [NBP2-79879] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.")
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