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ACAA2 Antibody (OTI1C10)

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Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; ...read more
Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2.
Western Blot: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of extracts (10ug) from 7 Human tissues ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon)
Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ACAA2.
Immunofluorescence: ACAA2 Antibody (OTI1C10) [NBP2-46477] - Analysis of HeLa cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71602AF350
Alexa Fluor 405
NBP2-71602AF405
Alexa Fluor 488
NBP2-71602AF488
Alexa Fluor 532
NBP2-71602AF532
Alexa Fluor 594
NBP2-71602AF594
Alexa Fluor 647
NBP2-71602AF647
Alexa Fluor 700
NBP2-71602AF700
Alexa Fluor 750
NBP2-71602AF750
Allophycocyanin
NBP2-71602APC
Biotin
NBP2-71602B
DyLight 350
NBP2-71602UV
DyLight 405
NBP2-71602V
DyLight 488
NBP2-71602G
DyLight 550
NBP2-71602R
DyLight 594
NBP2-71602DL594
DyLight 650
NBP2-71602C
DyLight 680
NBP2-71602FR
DyLight 755
NBP2-71602IR
FITC
NBP2-71602F
HRP
NBP2-71602H
Janelia Fluor 549
NBP2-71602JF549
Janelia Fluor 646
NBP2-71602JF646
PE
NBP2-71602PE
PerCP
NBP2-71602PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71602
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ACAA2 Antibody (OTI1C10) Summary

Immunogen
Full length human recombinant protein of human ACAA2(NP_006102) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ACAA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-4000
Theoretical MW
41.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ACAA2 Antibody (OTI1C10)

  • 3-ketoacyl-CoA thiolase, mitochondrial
  • acetyl-CoA acyltransferase 2
  • Acetyl-CoA acyltransferase
  • acetyl-Coenzyme A acyltransferase 2
  • beta ketothiolase
  • beta-ketothiolase
  • DSAEC
  • EC 2.3.1
  • EC 2.3.1.16
  • FLJ35992
  • FLJ95265
  • Mitochondrial 3-oxoacyl-CoA thiolase
  • mitochondrial 3-oxoacyl-Coenzyme A thiolase
  • T1

Background

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ACAA2