![Western Blot: Prohibitin Antibody (PHB/3194) [NBP3-07841] - Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).](http://images.novusbio.com/fullsize/Prohibitin-Antibody-PHB-3194-Western-Blot-NBP3-07841-img0005.jpg "Western Blot: Prohibitin Antibody (PHB/3194) [NBP3-07841] - Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, MA |
| Clone | PHB/3194 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08752) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Prohibitin protein fragment (aa167-261) (exact sequence is proprietary) (Uniprot: P35232) |
| Localization | Cytoplasmic (Mitochondria) |
| Marker | Mitochondrial Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PHB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Prohibitin Antibody (NBP3-07841)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (PHB/3194) [NBP3-07841] - Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194) labeled with CF488 (Green); RedDot is used to label the nuclei.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-PHB-3194-Immunocytochemistry-Immunofluorescence-NBP3-07841-img0002.jpg "Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (PHB/3194) [NBP3-07841] - Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194) labeled with CF488 (Green); RedDot is used to label the nuclei.")
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