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PRL-3/PTP4A3 Antibody (334402)

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat PRL-3 Monoclonal Antibody (Catalog # MAB3219) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRL-3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
334402
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3219-SP
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PRL-3/PTP4A3 Antibody (334402) Summary

Immunogen
E. coli-derived recombinant human PRL-3
Ala2-Met173
Accession # O75365
Specificity
Detects endogenous human, mouse and rat PRL-3 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PRL-1 or PRL‑2.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTP4A3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.3 ug/mL
Publications
Read Publications using
MAB3219 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRL-3/PTP4A3 Antibody (334402)

  • PRL3
  • PRL-3
  • PRL3EC 3.1.3.48
  • PRL-3potentially prenylated protein tyrosine phosphatase
  • PRL-Rprotein tyrosine phosphatase type IVA 3
  • protein tyrosine phosphatase type IVA, member 3
  • Protein-tyrosine phosphatase 4a3
  • Protein-tyrosine phosphatase of regenerating liver 3
  • PTP4A3

Background

Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRL-3/PTP4A3 Antibody (MAB3219)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(4)
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Human
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB3219 Applications Species
Jeffery T. Jolly, Ty C. Cheatham, Jessica S. Blackburn Phosphatase and Pseudo-Phosphatase Functions of Phosphatase of Regenerating Liver 3 (PRL-3) Are Insensitive to Divalent Metals In Vitro ACS Omega 2023-08-22 [PMID: 37636930]
Smith, CN;Kihn, K;Williamson, ZA;Chow, KM;Hersh, LB;Korotkov, KV;Deredge, D;Blackburn, JS; Development and characterization of nanobodies that specifically target the oncogenic Phosphatase of Regenerating Liver-3 (PRL-3) and impact its interaction with a known binding partner, CNNM3 PloS one 2023-05-23 [PMID: 37220097] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
John S. Lazo, Elizabeth R. Sharlow, Robert Cornelison, Duncan J. Hart, Danielle C. Llaneza, Anna J. Mendelson et al. Credentialing and Pharmacologically Targeting PTP4A3 Phosphatase as a Molecular Target for Ovarian Cancer Biomolecules 2021-06-30 [PMID: 34209460]
Trenkmann M, Brock M, Gay R, Michel B, Gay S, Huber L Tumor necrosis factor alpha-induced microRNA-18a activates rheumatoid arthritis synovial fibroblasts through a feedback loop in NF-kappaB signaling. Arthritis Rheum, 2013-04-01;65(4):916-27. 2013-04-01 [PMID: 23280137] (Western Blot, Human) Western Blot Human
Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Watanabe M Phosphatase of regenerating liver-3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma. Int. J. Cancer, 2010-08-01;127(3):543-54. 2010-08-01 [PMID: 19960436] (Western Blot, Human) Western Blot Human
Wang Z, He YL, Cai SR, Zhan WH, Li ZR, Zhu BH, Chen CQ, Ma JP, Chen ZX, Li W, Zhang LJ Expression and prognostic impact of PRL-3 in lymph node metastasis of gastric cancer: its molecular mechanism was investigated using artificial microRNA interference. Int. J. Cancer, 2008-09-15;123(6):1439-47. 2008-09-15 [PMID: 18561324] (IHC-P, Human) IHC-P Human
Daouti S, Li WH, Qian H, Huang KS, Holmgren J, Levin W, Reik L, McGady DL, Gillespie P, Perrotta A, Bian H, Reidhaar-Olson JF, Bliss SA, Olivier AR, Sergi JA, Fry D, Danho W, Ritland S, Fotouhi N, Heimbrook D, Niu H A selective phosphatase of regenerating liver phosphatase inhibitor suppresses tumor cell anchorage-independent growth by a novel mechanism involving p130Cas cleavage. Cancer Res., 2008-02-15;68(4):1162-9. 2008-02-15 [PMID: 18281492] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol PTP4A3
Uniprot