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PRKAG2 Antibody

Images

 
Western Blot: PRKAG2 Antibody [NBP1-89324] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/ Immunofluorescence: PRKAG2 Antibody [NBP1-89324] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human prostate shows moderate to strong positivity in nuclear membrane in glandular cells.
Western Blot: PRKAG2 Antibody [NBP1-89324] - Analysis in human cell line A-431.
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human skin shows moderate to strong positivity in nuclear membrane in keratinocytes
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.
Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human testis shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PRKAG2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: STPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAG2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PRKAG2 Protein (NBP1-89324PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-89324 in the following applications:

Publications
Read Publications using
NBP1-89324 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PRKAG2 Antibody

  • AAKG
  • AAKG2
  • AMPK gamma2
  • CMH6AMPK subunit gamma-2
  • H91620p
  • H91620pAMPK gamma2
  • PRKAG2
  • protein kinase, AMP-activated, gamma 2 non-catalytic subunit
  • WPWS5'-AMP-activated protein kinase subunit gamma-2

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRKAG2 Antibody (NBP1-89324)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
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Review for PRKAG2 Antibody (NBP1-89324) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-89324:
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WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot PRKAG2 NBP1-89324
Enlarge
4
reviewed by:
Verified Customer
WB Human 10/04/2020
View

Summary

ApplicationWestern Blot
Sample Tested293 whole cell lysate,293T cell lysate
SpeciesHuman
LotA117130

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PRKAG2 Antibody (NBP1-89324) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for PRKAG2 Antibody (NBP1-89324)

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Blogs on PRKAG2

There are no specific blogs for PRKAG2, but you can read our latest blog posts.
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Recent Reviews

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5
0
4
1
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2
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1
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Verified Customer
10/04/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol PRKAG2