PRAME Antibody (PRAME/6928R) - BSA Free Summary
Description |
Positive Controls: Human testis or melanoma.
Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required. |
Additional Information |
Recombinant Monoclonal Antibody |
Immunogen |
Recombinant fragment (around aa100-509) of human PRAME protein (exact sequence is proprietary) |
Localization |
Nucleus. Chromosome. Cytoplasm. Golgi apparatus. Cell membrane |
Isotype |
IgG Kappa |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
PRAME |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry-Paraffin 1-2 ug/ml
|
Application Notes |
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined. |
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
10mM PBS |
Preservative |
0.05% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for PRAME Antibody (PRAME/6928R) - BSA Free
Background
PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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