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PRAME Antibody (PRAME/6928R)

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Formalin-fixed, paraffin-embedded human testis stained with PRAME antibody (PRAME/6928R). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human testis stained with PRAME antibody (PRAME/6928R). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human melanoma stained with PRAME antibody (PRAME/6928R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
PRAME/6928R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20841AF350
Alexa Fluor 405
NBP3-20841AF405
Alexa Fluor 488
NBP3-20841AF488
Alexa Fluor 532
NBP3-20841AF532
Alexa Fluor 594
NBP3-20841AF594
Alexa Fluor 647
NBP3-20841AF647
Alexa Fluor 700
NBP3-20841AF700
Alexa Fluor 750
NBP3-20841AF750
Allophycocyanin
NBP3-20841APC
Biotin
NBP3-20841B
CoraFluor 1
NBP3-20841CL1
DyLight 350
NBP3-20841UV
DyLight 405
NBP3-20841V
DyLight 488
NBP3-20841G
DyLight 550
NBP3-20841R
DyLight 594
NBP3-20841DL594
DyLight 650
NBP3-20841C
DyLight 680
NBP3-20841FR
DyLight 755
NBP3-20841IR
FITC
NBP3-20841F
HRP
NBP3-20841H
Janelia Fluor 525
NBP3-20841JF525
Janelia Fluor 549
NBP3-20841JF549
Janelia Fluor 646
NBP3-20841JF646
Janelia Fluor 669
NBP3-20841JF669
PE
NBP3-20841PE
PerCP
NBP3-20841PCP
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP3-20841
Novus Biologicals offers 100% Guarantee

PRAME Antibody (PRAME/6928R) Summary

Description
Positive Controls: Human testis or melanoma.

Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (around aa100-509) of human PRAME protein (exact sequence is proprietary)
Localization
Nucleus. Chromosome. Cytoplasm. Golgi apparatus. Cell membrane
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
PRAME
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PRAME Antibody (PRAME/6928R)

  • cancer/testis antigen 130
  • MAPEOIP4CT130
  • melanoma antigen preferentially expressed in tumors
  • OIP-4
  • Opa-interacting protein 4
  • Opa-interacting protein OIP4
  • preferentially expressed antigen in melanoma
  • Preferentially expressed antigen of melanoma

Background

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRAME