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PPIL3 Antibody (OTI2E7) - Azide and BSA Free

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Western Blot: PPIL3 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73574] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPIL3.
Immunocytochemistry/ Immunofluorescence: PPIL3 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73574] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PPIL3.
Immunohistochemistry: PPIL3 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73574] - Analysis of Human tonsil tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

PPIL3 Antibody (OTI2E7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human PPIL3(NP_572028) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPIL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PPIL3 Antibody (OTI2E7) - Azide and BSA Free

  • Cyclophilin J
  • cyclophilin-like protein 3
  • Cyclophilin-like protein PPIL3
  • CyPJ
  • EC 5.2.1.8
  • peptidyl-prolyl cis-trans isomerase-like 3
  • peptidylprolyl cis-trans isomerase-like protein 3
  • peptidylprolyl isomerase (cyclophilin)-like 3
  • PPIase
  • PPIase-like protein 3
  • Rotamase PPIL3

Background

PPIL3 encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Transcript variants derived from alternative splicing and/or alternative polyadenylation exist; some of these variants encode different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPIL3