![Western Blot: POLR1C Antibody (3A5-H2) [H00009533-M01] - POLR1C monoclonal antibody (M01), clone 3A5-H2 Analysis of POLR1C expression in K-562.](http://images.novusbio.com/fullsize/POLR1C-Antibody-3A5-H2-Western-Blot-H00009533-M01-img0008.jpg "Western Blot: POLR1C Antibody (3A5-H2) [H00009533-M01] - POLR1C monoclonal antibody (M01), clone 3A5-H2 Analysis of POLR1C expression in K-562.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 3A5-H2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | POLR1C (AAH08863, 1 a.a. ~ 346 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAASQAVEEMRSRVVLGEFGVRNVHTTDFPGNYSGYDDAWDQDRFEKNFRVDVVHMDENSLEFDMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHDDILIAQLRPGQEIDLLMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPVEGEAAEELSRCFSPGVIEVQEVQGKKVARVANPRLDTFSREIFRNEKLKKVVRLARVRDHYIFSVESTGVLPPDVLVSEAIKVLMGKCRRFLDELDAVQMD |
| Localization | Nuclear |
| Specificity | POLR1C - polymerase (RNA) I polypeptide C, 30kDa |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POLR1C |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: POLR1C Antibody (3A5-H2) [H00009533-M01] - Analysis of monoclonal antibody to POLR1C on HeLa cell. Antibody concentration 7 ug/ml.](http://images.novusbio.com/fullsize/POLR1C-Antibody-3A5-H2-Immunocytochemistry-Immunofluorescence-H00009533-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: POLR1C Antibody (3A5-H2) [H00009533-M01] - Analysis of monoclonal antibody to POLR1C on HeLa cell. Antibody concentration 7 ug/ml.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")