![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0012.jpg "Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Func, ICC/IF, IHC, IP |
| Clone | 2G12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | PLK1 (AAH02369, 1 a.a. ~ 603 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS |
| Localization | Nuclear |
| Marker | Mitosis Marker |
| Specificity | PLK1 - polo-like kinase 1 (Drosophila) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PLK1 |
| Purity | Ascites |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IHC-P usage reported in scientific literature (PMID: 24722282). |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | Ascites |
| Publication using H00005347-M01 | Applications | Species |
|---|---|---|
| Werle K, Chen J, Xu HG et al. Liver kinase B1 regulates the centrosome via PLK1. Cell Death Dis 2014-04-11 [PMID: 24722282] (ICC/IF, WB, IHC-P, IP, Human) | ICC/IF, WB, IHC-P, IP | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for PLK1 Antibody (H00005347-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of monoclonal antibody to PLK1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Immunocytochemistry-Immunofluorescence-H00005347-M01-img0007.jpg "Immunocytochemistry/Immunofluorescence: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of monoclonal antibody to PLK1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - PLK1 monoclonal antibody (M01), clone 2G12 Analysis of PLK1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0010.jpg "Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - PLK1 monoclonal antibody (M01), clone 2G12 Analysis of PLK1 expression in Hela S3 NE.")
![Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Western-Blot-H00005347-M01-img0011.jpg "Western Blot: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.")
![Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Proximity-Ligation-Assay-H00005347-M01-img0005.jpg "Proximity Ligation Assay: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-Immunoprecipitation-H00005347-M01-img0008.jpg "Immunoprecipitation: PLK1 Antibody (2G12) [H00005347-M01] - Analysis of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.")
![ELISA: PLK1 Antibody (2G12) [H00005347-M01] - Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PLK1-Antibody-2G12-ELISA-H00005347-M01-img0013.jpg "ELISA: PLK1 Antibody (2G12) [H00005347-M01] - Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")