![Western Blot: Plasma Kallikrein/KLKB1 Antibody (JE31-29) [NBP3-32506] - Western blot analysis of Plasma Kallikrein/KLKB1 on human liver tissue lysates with Rabbit anti-Plasma Kallikrein/KLKB1 antibody (NBP3-32506) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32506) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32506_rabbit-plasma-kallikrein-klkb1-mab-je31-29-278202416285248.jpg "Western Blot: Plasma Kallikrein/KLKB1 Antibody (JE31-29) [NBP3-32506] - Western blot analysis of Plasma Kallikrein/KLKB1 on human liver tissue lysates with Rabbit anti-Plasma Kallikrein/KLKB1 antibody (NBP3-32506) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32506) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | JE31-29 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human Plasma Kallikrein/KLKB1 aa 589-638 / 638. (Uniprot: P03952) |
| Localization | Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KLKB1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 71 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KLKB1 |
![Immunohistochemistry: Plasma Kallikrein/KLKB1 Antibody (JE31-29) [NBP3-32506] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Plasma Kallikrein/KLKB1 antibody (NBP3-32506) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32506) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32506_rabbit-plasma-kallikrein-klkb1-mab-je31-29-298202414333669.jpg "Immunohistochemistry: Plasma Kallikrein/KLKB1 Antibody (JE31-29) [NBP3-32506] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Plasma Kallikrein/KLKB1 antibody (NBP3-32506) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32506) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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