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PKA R2 [p Ser99] Antibody (SR1905)

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Western Blot: PKA R2 [p Ser 99] Antibody (SR1905) [NBP3-21511] - Western blot analysis using NBP3-21511 in K562 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SR1905
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PKA R2 [p Ser99] Antibody (SR1905) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human phospho-PKA R2 [Ser99] (Uniprot #: P13861)
Modification
p Ser99
Specificity
Detects endogenous levels of total phospho-PKA R2 [Ser99]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRKAR2A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PKA R2 [p Ser99] Antibody (SR1905)

  • protein kinase, cAMP-dependent, regulatory, type II, alpha
  • RII-alpha subunit

Background

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKA R2 Antibody (NBP3-21511) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKAR2A