![Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunocytochemistry-Immunofluorescence-NBP2-67571-img0002.jpg "Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
| Reactivity | Hu, Mu, Rt, PoSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | SC54-04 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 aa 82-126 / 404. (SwissProt: P13861 Human; SwissProt: P12367 Mouse; SwissProt: P12368 Rat) |
| Modification | p Ser99 |
| Localization | Cell membrane, Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PRKAR2A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot PKLR Antibody [Unconjugated]](https://images.novusbio.com/images2/PKLR_AF8519_Western_Blot_16592.jpg)
![Simple Western PKLR Antibody [Unconjugated]](https://images.novusbio.com/images2/af8519_human-mouse-rat-pklr-affinity-purified-pab-simple-western-1342022154119.jpg)
![PKA R2 [p Ser99] Antibody (SC54-04)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "PKA R2 [p Ser99] Antibody (SC54-04)")
Secondary Antibodies |
Isotype Controls |
Research Areas for PKA R2 Antibody (NBP2-67571)Find related products by research area.
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| Gene Symbol | PRKAR2A |
![Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded mouse testis tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunohistochemistry-Paraffin-NBP2-67571-img0005.jpg "Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded mouse testis tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunocytochemistry-Immunofluorescence-NBP2-67571-img0001.jpg "Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded human spleen tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunohistochemistry-Paraffin-NBP2-67571-img0003.jpg "Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded human spleen tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded mouse heart tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunohistochemistry-Paraffin-NBP2-67571-img0004.jpg "Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded mouse heart tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.")
![Western Blot PKA RI beta Antibody [Unconjugated]](https://images.novusbio.com/images2/PKA_RI_beta_AF4177_Western_Blot_5414.jpg)
![Western Blot PKA RI beta Antibody [Unconjugated]](https://images.novusbio.com/images2/PKA_RI_beta_AF4177_Western_Blot_6318.jpg)
![Simple Western PKA RI beta Antibody [Unconjugated]](https://images.novusbio.com/images2/PKA_RI_beta_AF4177_Simple_Western_17508.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")