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PKA R2 [p Ser99] Antibody (S05-7G2)

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Western Blot: PKA R2 [p Ser99] Antibody (S05-7G2) [NBP3-19740] - Western blot detection of Phospho-PKA R2 (Ser99) in Rat Brain, C6 cell lysates using NBP3-19740 (1:1000 diluted). Predicted band size: 46kDa. Observed ...read more
Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (S05-7G2) [NBP3-19740] - Immunohistochemistry of PKA R2 (Phospho-Ser99) in paraffin-embedded Human tonsil using NBP3-19740 at dilution 1/100

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
S05-7G2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

PKA R2 [p Ser99] Antibody (S05-7G2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser99 of human PKA R2 (Uniprot # P13861)
Modification
p Ser99
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRKAR2A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/100
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for PKA R2 [p Ser99] Antibody (S05-7G2)

  • protein kinase, cAMP-dependent, regulatory, type II, alpha
  • RII-alpha subunit

Background

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKAR2A