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Pirh2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Pirh2 Antibody [NBP1-90098] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: Pirh2 Antibody [NBP1-90098] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Pirh2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHL
Predicted Species
Mouse (91%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCHY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Pirh2 Protein (NBP1-90098PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Pirh2 Antibody

  • Androgen receptor N-terminal-interacting protein
  • androgen-receptor N-terminal-interacting protein
  • ARNIPRING finger protein 199
  • CHIMPRNF199DKFZp586C1620
  • CH-rich interacting match with PLAG1
  • CH-rich-interacting match with PLAG1
  • E3 ubiquitin-protein ligase Pirh2
  • EC 6.3.2.-
  • hARNIP
  • hPirh2
  • PIRH2
  • PRO1996
  • ring finger and CHY zinc finger domain containing 1
  • RING finger and CHY zinc finger domain-containing protein 1
  • Zinc finger protein 363p53-induced RING-H2 protein
  • ZNF363

Background

Pirh2 encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RCHY1