![Western Blot: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.](http://images.novusbio.com/fullsize/PINCH1-LIMS1-Antibody-5G7-Western-Blot-NBP2-37581-img0003.jpg "Western Blot: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 5G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Purified recombinant fragment of human PINCH1/LIMS1 expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LIMS1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.03% Sodium Azide |
| Purity | Unpurified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LIMS1 |
![Immunocytochemistry/Immunofluorescence: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PINCH1-LIMS1-Antibody-5G7-Immunocytochemistry-Immunofluorescence-NBP2-37581-img0004.jpg "Immunocytochemistry/Immunofluorescence: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Flow Cytometry: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/PINCH1-LIMS1-Antibody-5G7-Flow-Cytometry-NBP2-37581-img0005.jpg "Flow Cytometry: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).")
![Western Blot: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)](http://images.novusbio.com/fullsize/PINCH1-LIMS1-Antibody-5G7-Western-Blot-NBP2-37581-img0002.jpg "Western Blot: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)")
![ELISA: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PINCH1-LIMS1-Antibody-5G7-ELISA-NBP2-37581-img0001.jpg "ELISA: PINCH1/LIMS1 Antibody (5G7) [NBP2-37581] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![N/A CD46 Antibody (344519) [Unconjugated]](https://images.novusbio.com/images/antibody/mab2005_human-cd46-mab-clone-344519-209202413163..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")