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PIK3CA Antibody (OTI6D9) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clone
OTI6D9
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

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PIK3CA Antibody (OTI6D9) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Human recombinant protein fragment corresponding to amino acids 303-631 of human PIK3CA (NP_006209) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PIK3CA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for PIK3CA Antibody (OTI6D9) [CoraFluor™ 1]

  • CLAPO
  • CLOVE
  • CWS5
  • EC 2.7.1
  • EC 2.7.1.153
  • MCMTC
  • MGC142161
  • MGC142163
  • P110alpha
  • p110-alpha
  • Phosphatidylinositol 3-Kinase catalytic subunit alpha
  • phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
  • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • PI3K
  • PI3Kalpha
  • PI3K-alpha
  • PI3-kinase p110 subunit alpha
  • PI3-kinase subunit alpha
  • PIK3CA
  • PtdIns-3-kinase p110
  • PtdIns-3-kinase subunit alpha
  • PtdIns-3-kinase subunit p110-alpha

Background

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional PIK3CA Products

Array NBP2-73406CL1

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Bioinformatics

Gene Symbol PIK3CA