![Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186] - Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospholamban. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/Phospholamban-[p-Ser16]-Antibody-Western-Blot-NB600-1186-img0001.jpg "Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186] - Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospholamban. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.")
| Reactivity | Hu, BvSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Description | May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Immunogen | Synthetic peptide corresponding to aa14-25, RA[pS]TIEMPQQAR-C of human cardiac phospholamban with a cysteine added at the C-terminal, conjugated to KLH. Species Sequence Homology: mouse, rat, rabbit, porcine and canine |
| Modification | p Ser16 |
| Localization | Membrane |
| Specificity | Recognizes bovine Phospho-lamban phosphorylated (Ser16) (5-25kD). Additional non-specific bands may be detected at 75 and 100kD in some lysates. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLN |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western Blot: 0.5-2ug/ml detects phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10ug PKA for 10 minutes. Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with NB600-1186(2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Phospholamban [p Ser16] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Phospholamban [p Ser16] Antibody")
Secondary Antibodies |
Isotype Controls |
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