PGLYRP3/PGRPIA Antibody (187C434)

Primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-1a (PGRP-3), and PGRP-1beta (PGRP-4)) define a new family of human pattern recognition molecules. PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood. PGRP-1a mRNA is highly expressed in esophagus, tonsils and thymus and to lesser extent in the stomach, rectum, and brain.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry Control, Flow-CS, ICC/IF.

Showing Publications 1 - 6 of 6.

Publications using NB100-56729	Applications	Species
Hua X, Yuan X, Li Z et al. A Novel Innate Response of Human Corneal Epithelium to Heat-killed Candida albicans by Producing Peptidoglycan Recognition Proteins PLoS ONE. 2015-06-04 [PMID: 26039076] (ICC/IF, Human) Details: PGLYRP4/PGRP-I beta antibody (clone 186C426; Imgenex IMG-414) was used for ICC-IF staining on human corneal epithelial cells /HCECs that were incubated in the absence or presence of isotype IgG (10ug/ml), dectin-1 neutralizing Ab (10ug/ml), BAY11-7082 (10uM) or NF-kB activation inhibitor quinazoline (NF-kB-I, 10uM) for 1 h followed by 48 hours incubation of HKCA/heat-killed Candida albicans (10^6 cells/ml). The immunoassay implicated 10 minutes RT fixation with 2% PFA, 10 minutes RT permeabilization with PBS-0.2% Triton X-100, detection of primary using Alexa-Fluor 488 conjugated secondary antibody (Fig 4A).	ICC/IF	Human
Ma P, Wang Z, Pflugfelder SC, Li DQ. Toll-like receptors mediate induction of peptidoglycan recognition proteins in human corneal epithelial cells. Exp Eye Res. 2010-01-01 [PMID: 19799901] (ICC/IF, Human) Details: PGRP-1alpha (IMG-391) & PGRP-1beta (IMG-414). IF/ICC: Human corneoscleral tissue & primary cultured human corneal epithelial cells, Fig 1.	ICC/IF	Human
Uehara A, Takada H. Synergism between TLRs and NOD1/2 in oral epithelial cells. J Dent Res. 2008-07-01 [PMID: 18573991] (Flow-CS) Details: flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), PGRP-S (IMG-393). Oral epithelial HSC-2 cell line stimulated with Fk156 or muramyldipeptide (MDP) plus FSL-1 and lipid A, Fig 1.	Flow-CS
Uehara A, Fujimoto Y, Fukase K, Takada H. Various human epithelial cells express functional Toll-like receptors, NOD1 and NOD2 to produce anti-microbial peptides, but not proinflammatory cytokines. Mol Immunol. 2007-05-01 [PMID: 17403538] (Flow-CS, Human) Details: The following products were used in flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), PGRP-S (IMG-393). Human oral epithelial (HSC-2, HSC-3, SAS, & HO-1-u-1), human pharyngeal epithelial (HEp-2), human esophageal epithelial (TE-1), human b	Flow-CS	Human
Uehara A, Fujimoto Y, Kawasaki A et al. Meso-diaminopimelic acid and meso-lanthionine, amino acids specific to bacterial peptidoglycans, activate human epithelial cells through NOD1. J Immunol. 2006-08-01 [PMID: 16849490] (Flow Cytometry Control) Details: flow (Intracellular): PGRP-1alpha (IMG-391) & PGRP-1beta (IMG-414); Human oral epithelial (HSC-2) cell line, Fig 2.	Flow Cytometry Control
Uehara A, Sugawara Y, Kurata S et al. Chemically synthesized pathogen-associated molecular patterns increase the expression of peptidoglycan recognition proteins via toll-like receptors, NOD1 and NOD2 in human oral epithelial cells. Cell Microbiol. 2005-05-01 [PMID: 15839897] (ICC/IF, Flow-CS) Details: Flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), and PGRP-S (IMG-393) were used in human oral epithelial (HSC-2) cell lines, Fig 3. IF/ICC: HSC-2 cells stimulated with or without lipid A, muramyldipeptide (MDP), gama-D-glutamyl-meso-DAP (iE-DAP), or IFN gamma then stained with PGRP-1beta (IMG-414), Fig 4.	ICC/IF, Flow-CS

Array NB100-56729

• PGLYRP3/PGRPIA Antibodies
• PGLYRP3/PGRPIA Lysate
• PGLYRP3/PGRPIA Proteins and Enzyme