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Recombinant Human PGK2 His Protein

Name: Recombinant Human PGK2 His Protein
Brand: Novus Biologicals
SKU: NBP2-52287
Price: 159 USD

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SDS-Page: PGK2 Recombinant Protein [NBP2-52287] - 15% SDS Page (3 ug)

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	PAGE, Enzyme Activity
Concentration	0.5 mg/ml

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Recombinant Human PGK2 His Protein Summary

Description	A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-417 of Human PGK2 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
Details of Functionality	Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
Source	E. coli
Protein/Peptide Type	Recombinant Protein
Gene	PGK2
Purity	>95%, by SDS-PAGE

Applications/Dilutions

Dilutions	Enzyme Activity SDS-Page
Theoretical MW	46.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Purity	>95%, by SDS-PAGE

Alternate Names for Recombinant Human PGK2 His Protein

dJ417L20.2
EC 2.7.2.3
PGK-2
PGKB
PGKPS
phosphoglycerate kinase 2
phosphoglycerate kinase autosomal pseudogene
Phosphoglycerate kinase, testis specific

Background

The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for PGK2 Recombinant Protein (NBP2-52287) (0)

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Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.

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NB300-221

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

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Bioinformatics

Gene Symbol

PGK2

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Recombinant Human PGK2 His Protein

Species Glossary

Images

Product Details

Order Details

Recombinant Human PGK2 His Protein Summary

Applications/Dilutions

Packaging, Storage & Formulations

Alternate Names for Recombinant Human PGK2 His Protein

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for PGK2 Recombinant Protein (NBP2-52287) (0)

Reviews for PGK2 Recombinant Protein (NBP2-52287) (0)

FAQs for PGK2 Recombinant Protein (NBP2-52287) (0)

Additional PGK2 Products

Research Areas for PGK2 Recombinant Protein (NBP2-52287)

Blogs on PGK2

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Contact Information

Product PDFs

Calculators

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Bioinformatics