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AKR1CL2 Antibody

Images

 
Western Blot: AKR1CL2 Antibody [NBP1-90195] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: AKR1CL2 Antibody [NBP1-90195] - Staining in human testis and liver tissues using anti-AKR1E2 antibody. Corresponding AKR1E2 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: AKR1CL2 Antibody [NBP1-90195] - Staining of human small intestine shows strong cytoplasmic positivity with granular pattern in glandular cells.
Immunohistochemistry-Paraffin: AKR1CL2 Antibody [NBP1-90195] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: AKR1CL2 Antibody [NBP1-90195] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

AKR1CL2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MGFKPPHPEWIMSCSELSFCLSHPRVQDLPLDESNMVIPSDTDFLDTWEAMEDLVITGLVKNIG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKR1E2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
AKR1CL2 Protein (NBP1-90195PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for AKR1CL2 Antibody

  • AF reductase
  • AKR1CL2
  • AKRDC1
  • Aldo-keto reductase family 1 member C-like protein 21,5-anhydro-D-fructose reductase
  • Aldo-keto reductase family 1 member E2
  • aldo-keto reductase family 1, member C-like 2
  • aldo-keto reductase family 1, member E2
  • aldo-keto reductase loopADR
  • aldo-keto reductase related protein
  • EC 1.1.1
  • EC 1.1.1.263
  • hTSP
  • LoopADR
  • MGC10612
  • Testis-specific protein

Background

AKR1CL2 catalyzes the oxidation of hydrogen sulfide, with the help of a quinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AKR1E2
Entrez
Uniprot