![Western Blot: Peripherin Antibody (8G2) [NB300-138] - Analysis of tissue and cell lysates probed with mouse mAb to peripherin, NB300-138, dilution 1:500 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] cow spinal cord, [5] SH-SY5Y cells, and [6] PC12 cells. The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.](http://images.novusbio.com/fullsize/Peripherin-Antibody-8G2-Western-Blot-NB300-138-img0002.jpg "Western Blot: Peripherin Antibody (8G2) [NB300-138] - Analysis of tissue and cell lysates probed with mouse mAb to peripherin, NB300-138, dilution 1:500 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] cow spinal cord, [5] SH-SY5Y cells, and [6] PC12 cells. The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 8G2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant rat Peripherin purified from E. coli. [UniProt# P21807] |
| Localization | Neuronal Marker |
| Marker | Un-myelinated Neurons Marker |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PRPH |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In WB a band can be seen at ~57 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Publication using NB300-138 | Applications | Species |
|---|---|---|
| Errante, L et al. J. Neurosci. Res. 37:515-528. 1994-01-01 [PMID: 8021973] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Peripherin Antibody (NB300-138)Find related products by research area.
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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera... Read full blog post. |
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A Look at Peripherin: The Unknown Filament The exact function of Peripherin, or Neurofilament 4, is unknown however it has been suggested to play a role in axon formation and determining and maintaining the shape of nerve cells. Peripherin is a 470 amino acid Class-III neuronal intermediate fi... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (8G2) [NB300-138] - Analysis of human neuroblastoma cell line SH-SY5Y (A) and rat pheochromocytoma cell line PC12 (B), stained with mouse mAb to peripherin, NB300-138, dilution 1:500, in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament (IF) subunit proteins is revealed by NB300-138 antibody in the perinuclear region in some SH-SY5Y cells and in all PC12 cells. Vimentin, a protein also in the Class III IF family, is detected in a subpopulation of SH-SY5Y cells which are negative for peripherin. PC12 cells express peripherin but not vimentin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-8G2-Immunocytochemistry-Immunofluorescence-NB300-138-img0001.jpg "Immunocytochemistry/Immunofluorescence: Peripherin Antibody (8G2) [NB300-138] - Analysis of human neuroblastoma cell line SH-SY5Y (A) and rat pheochromocytoma cell line PC12 (B), stained with mouse mAb to peripherin, NB300-138, dilution 1:500, in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament (IF) subunit proteins is revealed by NB300-138 antibody in the perinuclear region in some SH-SY5Y cells and in all PC12 cells. Vimentin, a protein also in the Class III IF family, is detected in a subpopulation of SH-SY5Y cells which are negative for peripherin. PC12 cells express peripherin but not vimentin.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")