Home » PDGF-C » PDGF-C Antibodies » PDGF-C Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

PDGF-C Antibody [Unconjugated]

 (7 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
PDGF‑C was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1447) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
PDGF‑C was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1447) at 5 µg/mL for 1 ...read more
Recombinant Mouse PDGF‑CC stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.8 µg/mL Recombinant Mouse PDGF‑CC is neutralized ...read more
FREM1 regulates activation of AKT and MAPK upon PDGFC stimulation. (A) Representative western blotting of phosphorylation of AKT and MAPK ERK1/2 in MEFs from WT and bat mouse embryos stimulated with PDGFCC for the ...read more
FREM1 regulates activation of AKT and MAPK upon PDGFC stimulation. (A) Representative western blotting of phosphorylation of AKT and MAPK ERK1/2 in MEFs from WT and bat mouse embryos stimulated with PDGFCC for the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1447
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1447-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

PDGF-C Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse PDGF-CC
Val235-Gly345
Accession # Q8CI19
Specificity
Detects mouse PDGF‑C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) PDGF-CC is observed and less than 1% cross-reactivity with rhPDGF-AA, rhPDGF-BB, rhPDGF-D, and recombinant rat PDGF-AB is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDGFC
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 4-16 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1447 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF-C Antibody [Unconjugated]

  • FALLOTEIN
  • PDGFC
  • PDGF-C
  • platelet derived growth factor C
  • platelet-derived growth factor C
  • SCDGFSpinal cord-derived growth factor
  • secretory growth factor-like protein
  • VEGF-E

Background

PDGF-C, also named spinal cord derived growth factor (SCDGF) and follotain, is a member of the PDGF family that binds to the PDGF receptor alpha alpha and alpha beta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(684 Publications)
Species: Hu
Applications: ELISA
PDGF-DD
[Unconjugated]
1159-SB
PDGF-DD [Unconjugated]
(18 Publications)
Species: Hu
Applications: BA
PDGF-AA
[Unconjugated]
221-AA
Bioactivity PDGF-AA [Unconjugated]
Cell Culture PDGF-AA [Unconjugated]
(57 Publications)
Species: Hu
Applications: BA
PDGF-B Antibody
NBP1-58279
Knockdown Validated PDGF-B Antibody
Immunocytochemistry/ Immunofluorescence PDGF-B Antibody
Western Blot PDGF-B Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
VEGFR1/Flt-1
Antibody [Unconjugate
AF321
Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]
Western Blot VEGFR1/Flt-1 Antibody [Unconjugated]
(2 Reviews)
(73 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
PLGF [HRP]
DPG00
N/A PLGF [HRP]
N/A PLGF [HRP]
N/A PLGF [HRP]
(79 Publications)
Species: Hu
Applications: ELISA
PDGF R beta
Antibody [Unconjugate
AF1042
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
Western Blot PDGF R beta Antibody [Unconjugated]
Immunohistochemistry PDGF R beta Antibody [Unconjugated]
(108 Publications)
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
PDGFR alpha
Antibody [Unconjugate
AF1062
Western Blot PDGFR alpha Antibody [Unconjugated]
Immunohistochemistry PDGFR alpha Antibody [Unconjugated]
Neutralization PDGFR alpha Antibody [Unconjugated]
(4 Reviews)
(298 Publications)
Species: Mu
Applications: Flow, IHC, Neut, WB
VEGFR2/KDR/Flk-1
Antibody [Unconjugate ...
AF644
Neutralization VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
(3 Reviews)
(98 Publications)
Species: Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Neut, WB
VEGF-C [HRP]
DVEC00
ELISA VEGF-C [HRP]
N/A VEGF-C [HRP]
N/A VEGF-C [HRP]
(54 Publications)
Species: Hu
Applications: ELISA
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(550 Publications)
Species: Hu
Applications: BA
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
VEGFR3/Flt-4
Antibody [Unconjugate
AF743
Flow Cytometry VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence VEGFR3/Flt-4 Antibody [Unconjugated]
(3 Reviews)
(140 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(49 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
PDGF-C Antibody
[Unconjugated]
AF1447
PDGF-C Antibody [Unconjugated]
Species: Mu
Applications: WB, IHC, B/N

Publications for PDGF-C Antibody (AF1447)(7)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: IHC, IHC-P, In Vivo, Neutralization, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
In Vivo
(1)
Neutralization
(2)
Western Blot
(3)
All Applications
Filter By Species
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF1447 Applications Species
JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression Science Advances, 2021-11-03;7(45):eabg9518. 2021-11-03 [PMID: 34730997] (IHC, In Vivo, Mouse) IHC, In Vivo Mouse
Wenjie Hu, Ruting Zhang, Wei Chen, Dongyue Lin, Kun Wei, Jiahui Li et al. Glycosylation at Asn254 Is Required for the Activation of the PDGF-C Protein Frontiers in Molecular Biosciences 5/24/2021 [PMID: 34109212]
L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently Oncotarget, 2016-11-22;7(47):77902-77915. 2016-11-22 [PMID: 27788490] (Neutralization, Western Blot, Mouse) Neutralization, Western Blot Mouse
Fenny Wiradjaja, Denny L. Cottle, Lynelle Jones, Ian Smyth Regulation of PDGFC signalling and extracellular matrix composition by FREM1 in mice Disease Models & Mechanisms 9/19/2013 [PMID: 24046351]
Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. J. Exp. Med., 2010-03-15;207(4):867-80. 2010-03-15 [PMID: 20231377] (Western Blot, Mouse) Western Blot Mouse
Crawford Y, Kasman I, Yu L, Zhong C, Wu X, Modrusan Z, Kaminker J, Ferrara N PDGF-C mediates the angiogenic and tumorigenic properties of fibroblasts associated with tumors refractory to anti-VEGF treatment. Cancer Cell, 2009-01-06;15(1):21-34. 2009-01-06 [PMID: 19111878] (Neutralization, Western Blot, Mouse) Neutralization, Western Blot Mouse
Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury. Lab. Invest., 2008-07-28;88(10):1090-100. 2008-07-28 [PMID: 18663351] (IHC-P, Rat) IHC-P Rat

Reviews for PDGF-C Antibody (AF1447) (0)

There are no reviews for PDGF-C Antibody (AF1447). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PDGF-C Antibody (AF1447) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PDGF-C Products

Blogs on PDGF-C

There are no specific blogs for PDGF-C, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PDGF-C Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol PDGFC
Uniprot