Recombinant Human PDGF-DD Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

The platelet-derived growth factor (PDGF) family consists of four disulfide-linked homodimers and one heterodimer (PDGF-AB). These proteins regulate diverse cellular functions through interactions with PDGF R alpha and R beta (1, 2). Mature PDGF-DD associates with PDGF R beta and triggers signaling through PDGF R beta homodimers and PDGF R alpha / beta heterodimers (3 - 5). The human PDGF-DD cDNA encodes a 370 amino acid (aa) precursor that includes a 23 aa signal sequence, one CUB domain, and one PDGF/VEGF domain (3, 4). The PDGF/VEGF domain shares 27 - 35% aa sequence identity with the corresponding regions of other PDGF family members. Human PDGF-DD shares 87% aa sequence identity with mouse and rat PDGF-DD. PDGF-DD is secreted as a100 kDa latent homodimer which is activated by proteolysis to release a 35 kDa bioactive protein containing the PDGF/VEGF homology domain (3 ,4 ,6 ,7). A splice variant of PDGF-DD has a 6 aa deletion near the N-terminus. A 72 aa deletion within the PDGF/VEGF domain generates an inactive protein in mouse but has not been detected in human (8). PDGF-DD is widely expressed in embryonic and adult tissues (3, 9, 10), and PDGF R beta is expressed in a generally complementary pattern (9, 11, 12). PDGF-DD functions as a growth factor for renal artery smooth muscle cells and lens epithelial cells, and as a macrophage chemoattractant (5, 9 - 11). PDGF-DD is overexpressed in and contributes to several disease states, including renal and hepatic fibrosis, mesangial proliferative glomerulopathy, pulmonary lymphoid infiltration, and many cancers (6, 11 - 15). PDGF-DD functions in both paracrine and autocrine manners (6, 7, 14).

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 6 applications: Bioassay, Cell Culture, In Vivo, Surface Plasmon Resonance, Western Blot, cell cutlure.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using 1159-SB	Applications	Species
Wong, KG;Cheng, YF;Wu, VH;Kiseleva, AA;Li, J;Poleshko, A;Smith, CL;Epstein, JA; Growth factor-induced activation of MSK2 leads to phosphorylation of H3K9me2S10 and corresponding changes in gene expression Science advances 2024-03-15 [PMID: 38478612] (Bioassay, Human)	Bioassay	Human
HJ Kim, P Cheng, S Travisano, C Weldy, JP Monteiro, R Kundu, T Nguyen, D Sharma, H Shi, Y Lin, B Liu, S Haldar, S Jackson, T Quertermou Molecular mechanisms of coronary artery disease risk at the PDGFD locus Nature Communications, 2023-02-15;14(1):847. 2023-02-15 [PMID: 36792607] (Bioassay, Human)	Bioassay	Human
T Akiyama, T Yasuda, T Uchihara, N Yasuda-Yos, BJY Tan, A Yonemura, T Semba, J Yamasaki, Y Komohara, K Ohnishi, F Wei, L Fu, J Zhang, F Kitamura, K Yamashita, K Eto, S Iwagami, H Tsukamoto, T Umemoto, M Masuda, O Nagano, Y Satou, H Saya, P Tan, H Baba, T Ishimoto Stromal reprogramming through dual PDGFRalpha/beta blockade boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors Cancer Research, 2023-03-02;0(0):. 2023-03-02 [PMID: 36543251] (Bioassay, Human)	Bioassay	Human
T Pham, AJ Najy, HC Kim E3 ligase HUWE1 promotes PDGF D-mediated osteoblastic differentiation of mesenchymal stem cells by effecting polyubiquitination of beta-PDGFR The Journal of Biological Chemistry, 2022-04-25;0(0):101981. 2022-04-25 [PMID: 35472332] (Bioassay, Human)	Bioassay	Human
Z Li, S Yu, Y Liu, X Hu, Y Li, Z Xiao, Y Chen, D Tian, X Xu, L Cheng, M Zheng, J Jing SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRbeta pathway Journal of Neuroinflammation, 2022-04-16;19(1):95. 2022-04-16 [PMID: 35429978] (In Vivo, Mouse)	In Vivo	Mouse
JO Kim, SE Baek, EY Jeon, JM Choi, EJ Jang, CD Kim PDGFR-beta signaling mediates HMGB1 release in mechanically stressed vascular smooth muscle cells PLoS ONE, 2022-03-16;17(3):e0265191. 2022-03-16 [PMID: 35294955] (cell cutlure, Rat)	cell cutlure	Rat
Y Yamamoto, A Mukai, T Ikushima, Y Urata, S Kinoshita, J Hamuro, M Ueno, C Sotozono Pluripotent epigenetic regulator OBP-801 maintains filtering blebs in glaucoma filtration surgery model Scientific Reports, 2020-12-01;10(1):20936. 2020-12-01 [PMID: 33262357] (Cell Culture, Human)	Cell Culture	Human
SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017-11-27;7(1):16439. 2017-11-27 [PMID: 29180757] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
Integrative functional genomics identifies regulatory mechanisms at coronary artery disease loci Nat Commun, 2016-07-08;7(0):12092. 2016-07-08 [PMID: 27386823] (Bioassay, Human)	Bioassay	Human
Heim E, Marston J, Federman R, Edwards A, Karabadzhak A, Petti L, Engelman D, DiMaio D Biologically active LIL proteins built with minimal chemical diversity. Proc Natl Acad Sci U S A, 2015-08-10;112(34):E4717-25. 2015-08-10 [PMID: 26261320] (Bioassay, Human)	Bioassay	Human
Obenauf A, Zou Y, Ji A, Vanharanta S, Shu W, Shi H, Kong X, Bosenberg M, Wiesner T, Rosen N, Lo R, Massague J Therapy-induced tumour secretomes promote resistance and tumour progression. Nature, 2015-03-25;520(7547):368-72. 2015-03-25 [PMID: 25807485] (Bioassay, Human)	Bioassay	Human
Petti L, Talbert-Slagle K, Hochstrasser M, DiMaio D A single amino acid substitution converts a transmembrane protein activator of the platelet-derived growth factor beta receptor into an inhibitor. J Biol Chem, 2013-08-01;288(38):27273-86. 2013-08-01 [PMID: 23908351] (Bioassay, Human)	Bioassay	Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Martino MM, Hubbell JA The 12th-14th type III repeats of fibronectin function as a highly promiscuous growth factor-binding domain. FASEB J., 2010-07-29;24(12):4711-21. 2010-07-29 [PMID: 20671107] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
Crawford Y, Kasman I, Yu L, Zhong C, Wu X, Modrusan Z, Kaminker J, Ferrara N PDGF-C mediates the angiogenic and tumorigenic properties of fibroblasts associated with tumors refractory to anti-VEGF treatment. Cancer Cell, 2009-01-06;15(1):21-34. 2009-01-06 [PMID: 19111878] (Western Blot)	Western Blot
Rice JW, Veal JM, Fadden RP, Barabasz AF, Partridge JM, Barta TE, Dubois LG, Huang KH, Mabbett SR, Silinski MA, Steed PM, Hall SE Small molecule inhibitors of Hsp90 potently affect inflammatory disease pathways and exhibit activity in models of rheumatoid arthritis. Arthritis Rheum., 2008-12-01;58(12):3765-75. 2008-12-01 [PMID: 19035474] (Bioassay, Mouse)	Bioassay	Mouse
Wagsater D, Zhu C, Bjorck HM, Eriksson P Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression. Atherosclerosis, 2008-05-22;202(2):415-23. 2008-05-22 [PMID: 18573494] (Bioassay, Human)	Bioassay	Human
McCabe KL, Bronner-Fraser M Essential role for PDGF signaling in ophthalmic trigeminal placode induction. Development, 2008-04-16;135(10):1863-74. 2008-04-16 [PMID: 18417621] (In Vivo, Chicken)	In Vivo	Chicken
Show All 18 Publications. Collapse Publications.

Array 1159-SB

• PDGF-DD ELISAs
• PDGF-DD Proteins and Enzymes