Recombinant Human PDGF-B His Protein Summary
Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 82-190 of Human PDGF-B Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCKTRTEVFE ISRRLIDRTN ANFLVWPPCV EVQRCSGCCN NRNVQCRPTQ VQLRPVQVRK IEIVRKKPIF KKATVTLEDH LACKCETVAA ARPVTRSPGG SQEQRAKTPQ |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
PDGFB |
Purity |
>95%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
14.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.4), 10% glycerol |
Preservative |
No Preservative |
Concentration |
0.25 mg/ml |
Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant Human PDGF-B His Protein
Background
Platelet-Derived Growth Factor-BB (PDGF-BB) is a disulfide-linked homodimer of two B-chains (109 aa each). It is stored in platelet granules and is released folllowing activation of platelets. PDGF-BB has potent mitogenic effects on various cell types such as cells of connective tissue, bone and cartilage and smooth muscle. It promotes bone formation and soft tissue repair, stimulating wound healing processes. PDGF-BB is involved in the development of the nervous system and may act as a neuronal regulatory agent via nerve regeneration and proliferation. It also plays a role in the epithelial cell development of the respiratory system, cell migration and angiogenesis. Benefits of NovActive Proteins, Fully Active, Fully folded naturally by eukaryotic source. Comparable for eukaryotic glycosylation BioRisk Free, animal-free, serum-free, endotoxin-free, infection reagent-free, low in proteolyic activity, low in pyrogenic and pro-inflammatory activity. Just reconstitute and add to your cell culture. Have FDA G.R.A.S. (generally recognized as safe) approval. Advantages of barley endosperm, Folding, Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment, A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status. Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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