This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)
Localization
Nuclear
Marker
Rhabdomyosarcoma Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PAX7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Alternate Names for Pax7 Antibody (PAX7/497) [Allophycocyanin]
FLJ37460
HUP1
paired box 7
paired box gene 7
paired box homeotic gene 7
paired box protein Pax-7
paired domain gene 7
PAX7 transcriptional factor
Pax7
PAX7B
RMS2
Background
PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Sample Information Cell Lines Tested: mouse skeletal muscle-derived primary cell population Species: mouse Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 Fixation Solution and Conditions: 4% formaldehyde treatment for 20min at RT
Blocking Solution: no-treatment because of using fluorescence conjugated antibody in this experiment Time and Temperature:
Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times
Primary Antibody Diluent: 0.5% tritonX-100 in PBS Dilutions Tested: 1:50 Time and Temperature: overnight at 4℃ Washing Wash Buffer: PBS Time: 5min Repetitions: 2 times
Detection System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 633nm Emission Filter: 691nm
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