What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?

Name: Pax6 Antibody (SPM612) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-11573
Price: 519 USD

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Accepted Answer

By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow, ICC/IF, IHC, ICC/IF
Clone	SPM612
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	Azide and BSA Free
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP3-11573AF350

Alexa Fluor 405 NBP3-11573AF405

Alexa Fluor 488 NBP3-11573AF488

Alexa Fluor 532 NBP3-11573AF532

Alexa Fluor 594 NBP3-11573AF594

Alexa Fluor 647 NBP3-11573AF647

Alexa Fluor 700 NBP3-11573AF700

Alexa Fluor 750 NBP3-11573AF750

Allophycocyanin NBP3-11573APC

Biotin NBP3-11573B

CoraFluor 1 NBP3-11573CL1

DyLight 350 NBP3-11573UV

DyLight 405 NBP3-11573V

DyLight 488 NBP3-11573G

DyLight 550 NBP3-11573R

DyLight 594 NBP3-11573DL594

DyLight 650 NBP3-11573C

DyLight 680 NBP3-11573FR

DyLight 755 NBP3-11573IR

FITC NBP3-11573F

HRP NBP3-11573H

Janelia Fluor 525 NBP3-11573JF525

Janelia Fluor 549 NBP3-11573JF549

Janelia Fluor 585 NBP3-11573JF585

Janelia Fluor 635 NBP3-11573JF635

Janelia Fluor 646 NBP3-11573JF646

Janelia Fluor 669 NBP3-11573JF669

PE NBP3-11573PE

PerCP NBP3-11573PCP

Unconjugated NBP2-44577-0.1mg

mFluor Violet 450 SE NBP3-11573MFV450

mFluor Violet 500 SE NBP3-11573MFV500

mFluor Violet 610 SE NBP3-11573MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NBP2-44577-0.1mg

IHC-Prediluted NBP2-48247

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-11573AF350
Alexa Fluor 405	NBP3-11573AF405
Alexa Fluor 488	NBP3-11573AF488
Alexa Fluor 532	NBP3-11573AF532
Alexa Fluor 594	NBP3-11573AF594
Alexa Fluor 647	NBP3-11573AF647
Alexa Fluor 700	NBP3-11573AF700
Alexa Fluor 750	NBP3-11573AF750
Allophycocyanin	NBP3-11573APC
Biotin	NBP3-11573B
CoraFluor 1	NBP3-11573CL1
DyLight 350	NBP3-11573UV
DyLight 405	NBP3-11573V
DyLight 488	NBP3-11573G
DyLight 550	NBP3-11573R
DyLight 594	NBP3-11573DL594
DyLight 650	NBP3-11573C
DyLight 680	NBP3-11573FR
DyLight 755	NBP3-11573IR
FITC	NBP3-11573F
HRP	NBP3-11573H
Janelia Fluor 525	NBP3-11573JF525
Janelia Fluor 549	NBP3-11573JF549
Janelia Fluor 585	NBP3-11573JF585
Janelia Fluor 635	NBP3-11573JF635
Janelia Fluor 646	NBP3-11573JF646
Janelia Fluor 669	NBP3-11573JF669
PE	NBP3-11573PE
PerCP	NBP3-11573PCP
Unconjugated	NBP2-44577-0.1mg
mFluor Violet 450 SE	NBP3-11573MFV450
mFluor Violet 500 SE	NBP3-11573MFV500
mFluor Violet 610 SE	NBP3-11573MFV610

Pax6 Antibody (SPM612) - Azide and BSA Free

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Description	1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44577). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen	Recombinant fragment (N-terminus; aa 1-300) of human Pax6 protein (Uniprot: P26367)
Localization	Nuclear
Marker	Stem Cell Marker
Isotype	IgG1 Kappa
Clonality	Monoclonal
Host	Mouse
Gene	PAX6
Purity	Protein A or G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml Immunocytochemistry/ Immunofluorescence 1-2 ug/ml Immunofluorescence 0.5 - 1.0 ug/ml Immunohistochemistry Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes	Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW	47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer	10 mM PBS
Preservative	No Preservative
Concentration	1 mg/ml
Purity	Protein A or G purified