I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).

Name: PARP Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF-600-NA
Price: 489 USD

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For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, MuSpecies Glossary
Applications	WB, Simple Western, IP, ICC/IF, KO
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF600

Catalog# & Formulation	Size	Price
Carrier Free AF-600-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF600

PARP Antibody [Unconjugated]

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Publications for PARP Antibody (AF-600-NA)(6)

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Immunogen	E. coli-derived recombinant mouse PARP Val71-Pro329 Accession # NP_031441
Specificity	Detects human and mouse PARP in Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	PARP1
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF-600-NA	Applications	Species
P Bontempo, P Stiuso, S Lama, A Napolitano, S Piacente, L Altucci, AM Molinari, L De Masi, D Rigano Metabolite Profile and In Vitro Beneficial Effects of Black Garlic (Allium sativum L.) Polar Extract Nutrients, 2021-08-13;13(8):. 2021-08-13 [PMID: 34444931] (Western Blot, Human)	Western Blot	Human
A Povo-Retan, M Mojena, AB Stremtan, VB Fernández-, A Gómez-Sáez, C Nuevo-Tapi, JM Molina-Gui, J Avendaño-O, JM Cuezva, E López-Coll, JF Martínez-L, L Boscá Specific Effects of Trabectedin and Lurbinectedin on Human Macrophage Function and Fate-Novel Insights Cancers (Basel), 2020-10-20;12(10):. 2020-10-20 [PMID: 33092171] (Western Blot, Human)	Western Blot	Human
L De Masi, P Bontempo, D Rigano, P Stiuso, V Carafa, A Nebbioso, S Piacente, P Montoro, R Aversano, V D'Amelia, D Carputo, L Altucci Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different Solanum tuberosum Varieties Molecules, 2020-01-06;25(1):. 2020-01-06 [PMID: 31935970] (Western Blot, Human)	Western Blot	Human
Nakajima H, Kubo T, Ihara H, Hikida T, Danjo T, Nakatsuji M, Shahani N, Itakura M, Ono Y, Azuma Y, Inui T, Kamiya A, Sawa A, Takeuchi T Nuclear-translocated Glyceraldehyde-3-phosphate Dehydrogenase Promotes Poly(ADP-ribose) Polymerase-1 Activation during Oxidative/Nitrosative Stress in Stroke. J Biol Chem, 2015-04-16;290(23):14493-503. 2015-04-16 [PMID: 25882840] (Western Blot, Human)	Western Blot	Human
Phillips G, Fields C, Li G, Dowbenko D, Schaefer G, Miller K, Andre F, Burris H, Albain K, Harbeck N, Dieras V, Crivellari D, Fang L, Guardino E, Olsen S, Crocker L, Sliwkowski M Dual targeting of HER2-positive cancer with trastuzumab emtansine and pertuzumab: critical role for neuregulin blockade in antitumor response to combination therapy. Clin Cancer Res, 2013-10-04;20(2):456-68. 2013-10-04 [PMID: 24097864] (Western Blot, Human)	Western Blot	Human
Temme A, Rodriguez JA, Hendruschk S, Gunes S, Weigle B, Schakel K, Schmitz M, Bachmann M, Schackert G, Rieber EP Nuclear localization of Survivin renders HeLa tumor cells more sensitive to apoptosis by induction of p53 and Bax. Cancer Lett., 2006-11-07;250(2):177-93. 2006-11-07 [PMID: 17084966] (Western Blot, Human)	Western Blot	Human

Gene Symbol	PARP1
Uniprot	NP_031441()