PARP Antibodies
We offer Poly (ADP-ribose) polymerase (PARP) Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each PARP Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PARP Antibodies can be used in a variety of model species: Drosophila, Human, Mouse, Multi-Species, Rat, Xenopus. We offer pre-conjugated 53BP1 Antibodies in Alexa Fluor®, Biotin, Dylight, FITC, HRP, PE and PerCP formats. Use the list below to choose the PARP Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PARP polyclonal antibodies and browse our PARP monoclonal antibody catalog.
Alternate Names for PARP Antibodies
anti-PARP antibody, anti-PARP1 antibody, anti-ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) antibody, anti-ADPRT 1 antibody, anti-ADPRTADP-ribosyltransferase NAD(+) antibody, anti-EC 2.4.2 antibody, anti-EC 2.4.2.30 antibody, anti-NAD(+) ADP-ribosyltransferase 1 antibody, anti-PARP apoptosis antibody, anti-PARP-1 antibody, anti-PARPADPRT1 antibody, anti-poly (ADP-ribose) polymerase 1 antibody, anti-poly (ADP-ribose) polymerase family, member 1 antibody, anti-poly [ADP-ribose] polymerase 1 antibody, anti-poly(ADP-ribose) polymerase antibody, anti-poly(ADP-ribose) synthetase antibody, anti-poly(ADP-ribosyl)transferase antibody, anti-Poly[ADP-ribose] synthase 1 antibody, anti-PPOLpADPRT-1 antibody
13 Results for "parp" in Primary Antibodies
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/fullsize/PARP-Antibody-194C1439-Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP2-13732-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP2-13732] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13732-img0007.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0014.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0010.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human pancreas shows strong nuclear positivity.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0011.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human parathyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0012.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0013.jpg)
![Western Blot: PARP Antibody (CL2220) [NBP2-36747] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Western-Blot-NBP2-36747-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody (CL2220) [NBP2-36747] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in U251 cell line showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0012.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in HeLa cell line showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining of human cell line A431 showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in MCF7 cell line showing clear cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (CL2220) [NBP2-36747] - Staining in U2OS cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunocytochemistry-Immunofluorescence-NBP2-36747-img0011.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human tonsil shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0015.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0016.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2220) [NBP2-36747] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2220-Immunohistochemistry-Paraffin-NBP2-36747-img0017.jpg)
![Western Blot: PARP Antibody (CL2209) [NBP2-36748] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Western-Blot-NBP2-36748-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody (CL2209) [NBP2-36748] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human liver shows nuclear immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0012.jpg)
![Western Blot: PARP Antibody (CL2209) [NBP2-36748] - Lane 1: Marker [kDa]. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Western-Blot-NBP2-36748-img0006.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human skin shows strong nuclear immunoreactivity in both epidermis and dermis.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0008.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human cerebellum shows strong nuclear positivity in granular and molecular layer cells, as well as in Purkinje cells.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0009.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human placenta shows strong nuclear immunoreactivity in trophoblast.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0010.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (CL2209) [NBP2-36748] - Staining of human kidney shows nuclear positivity in both tubules and glomeruli.](http://images.novusbio.com/fullsize/PARP-Antibody-CL2209-Immunohistochemistry-Paraffin-NBP2-36748-img0011.jpg)
![Western Blot: PARP Antibody (2O6H4) [NBP3-15802] - Western blot analysis of extracts of Jurkat cells, using PARP antibody (NBP3-15802) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PARP-Antibody-2O6H4-Western-Blot-NBP3-15802-img0004.jpg "Western Blot: PARP Antibody (2O6H4) [NBP3-15802] - Western blot analysis of extracts of Jurkat cells, using PARP antibody (NBP3-15802) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: PARP Antibody (2O6H4) [NBP3-15802] - Immunohistochemistry of paraffin-embedded mouse spleen using PARP antibody (NBP3-15802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PARP-Antibody-2O6H4-Immunohistochemistry-Paraffin-NBP3-15802-img0003.jpg)
![Western Blot: PARP Antibody (2O6H4) [NBP3-15802] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PARP-Antibody-2O6H4-Western-Blot-NBP3-15802-img0005.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (2O6H4) [NBP3-15802] - Immunohistochemistry of paraffin-embedded human appendix using PARP antibody (NBP3-15802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PARP-Antibody-2O6H4-Immunohistochemistry-Paraffin-NBP3-15802-img0001.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody (2O6H4) [NBP3-15802] - Immunohistochemistry of paraffin-embedded rat spleen using PARP antibody (NBP3-15802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PARP-Antibody-2O6H4-Immunohistochemistry-Paraffin-NBP3-15802-img0002.jpg)
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-262202415475644.jpg "Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-2722024850467.jpg)
![Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 KDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-282202411485824.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (8C7) [NBP3-26439] - Staining of Hela Cells with PARP Antibody (8C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241323611.jpg)
![Flow Cytometry: PARP Antibody (8C7) [NBP3-26439] - Overlay histogram showing Jurkat cells stained with PARP Antibody (8C7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241431270.jpg)
![Western Blot: PARP Antibody [NBP3-03817] - Analysis of extracts of various cell lines, using PARP1 antibody at 1:3000 dilution.HeLa cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP3-03817-img0010.jpg "Western Blot: PARP Antibody [NBP3-03817] - Analysis of extracts of various cell lines, using PARP1 antibody at 1:3000 dilution.HeLa cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-03817] - Analysis of U-2 OS cells using [KO Validated] PARP1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03817-img0013.jpg)
![Western Blot: PARP Antibody [NBP3-03817] - Analysis of extracts of various cell lines, using PARP1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP3-03817-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-03817] - Analysis of U-2 OS cells using PARP antibody at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03817-img0001.jpg)
![Western Blot: PARP Antibody [NBP3-09070] - Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP3-09070-img0001.jpg "Western Blot: PARP Antibody [NBP3-09070] - Western blot analysis of PARP1 on Daudi and 293 cell lysates using anti- PARP1 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-09070] - ICC staining PARP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-09070-img0003.jpg)
![Flow Cytometry: PARP Antibody [NBP3-09070] - Flow cytometric analysis of K562 cells with PARP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PARP-Antibody-Flow-Cytometry-NBP3-09070-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP3-09070] - ICC staining PARP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-09070-img0002.jpg)
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-262202415355711.jpg "Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (7C11) [NBP3-26438] - Image of PARP Antibody (7C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-272202485637.jpg)
![Western Blot: PARP Antibody (7C11) [NBP3-26438] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 kDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26438_rabbit-parp-mab-7c11-282202411472110.jpg)
![Western Blot: PARP Antibody (4M9X4) - Cleaved [NBP3-15813] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15813) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PARP-Antibody-4M9X4-Cleaved-Western-Blot-NBP3-15813-img0001.jpg "Western Blot: PARP Antibody (4M9X4) - Cleaved [NBP3-15813] - Western blot analysis of extracts of various cell lines, using PARP antibody (NBP3-15813) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")