![Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in paraffin embedded human placenta tissue.](http://images.novusbio.com/fullsize/Pappalysin-1-PAPP-A-Antibody-NA-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05116-img0002.jpg "Immunohistochemistry-Paraffin: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in paraffin embedded human placenta tissue.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Purified human PAPP-A |
| Epitope | The epitope is located on the PAPP-A subunit of the heterotetramer. |
| Specificity | Specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PAPPA |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: In sandwich ELISA the optimal combination is NBP1-05116 used as coating antibody and biotinylated NBP1-05043 used as detection antibody. NBP1-05116 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat.
WB: NBP1-05116 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of NBP2-21614 and NBP1-05043.
IHC: NBP1-05116 can be used in IHC. |
| Storage | Store at 4C in the dark. |
| Buffer | 10mM Phosphate (pH 7.4) and 0.5M NaCl |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Pappalysin-1/PAPP-A Antibody (NBP1-05116)Find related products by research area.
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| Gene Symbol | PAPPA |
![Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in frozen human placenta tissue.](http://images.novusbio.com/fullsize/Pappalysin-1-PAPP-A-Antibody-NA-BSA-Free-Immunohistochemistry-Frozen-NBP1-05116-img0001.jpg "Immunohistochemistry-Frozen: Pappalysin-1/PAPP-A Antibody (NA) - BSA Free [NBP1-05116] - Staining is restricted to the syncytiotrophoblast of the chronic villi in frozen human placenta tissue.")
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