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PAPD1 Antibody

Images

 
Western Blot: PAPD1 Antibody [NBP2-19690] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: PAPD1 Antibody [NBP2-19690] - Analysis of methanol-fixed HeLa, using Mt PAP(GTX118738) antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PAPD1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PAPD1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTPAP
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000
Application Notes
IP-Assay dependent
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PAPD1 Antibody

  • EC 2.7.7.19
  • FLJ10486
  • mitochondrial poly(A) polymerase
  • mtPAP
  • PAP associated domain containing 1
  • PAP
  • PAP-associated domain-containing protein 1
  • PAPD1SPAX4
  • poly(A) RNA polymerase, mitochondrial
  • Polynucleotide adenylyltransferase
  • Terminal uridylyltransferase 1
  • TUTase 1

Background

The protein encoded by this gene is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MTPAP
Uniprot