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![Immunocytochemistry/Immunofluorescence: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of methanol-fixed HeLa, using mtPAP antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PAPD1-Antibody-1D3-Immunocytochemistry-Immunofluorescence-NBP2-43686-img0001.jpg "Immunocytochemistry/Immunofluorescence: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of methanol-fixed HeLa, using mtPAP antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of Hepatoma, using mtPAP antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PAPD1-Antibody-1D3-Immunohistochemistry-Paraffin-NBP2-43686-img0002.jpg)
![Western Blot: PAPD1 Antibody (1D3) [NBP2-43686] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with mtPAP antibody [1D3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-43686_mouse-monoclonal-papd1-antibody-1d3-western-blot-66202394619..jpg)
![Western Blot: PAPD1 Antibody [NBP2-19690] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Western-Blot-NBP2-19690-img0001.jpg "Western Blot: PAPD1 Antibody [NBP2-19690] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: PAPD1 Antibody [NBP2-19690] - Analysis of methanol-fixed HeLa, using Mt PAP(GTX118738) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19690-img0002.jpg)
![Western Blot: PAPD1 Antibody [NBP2-22258] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Western-Blot-NBP2-22258-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: PAPD1 Antibody [NBP2-22258] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: PAPD1 Antibody [NBP2-22258] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22258 used for IP at 6 ug/mg lysate. PAPD1 was also immunoprecipitated by another rabbit anti-PAPD1 antibody. For blotting immunoprecipitated PAPD1, NBP2-22258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Immunoprecipitation-NBP2-22258-img0004.jpg)
![Western Blot: PAPD1 Antibody [NBP2-19689] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mt PAP antibody [N1C1] diluted at 1:3000.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Western-Blot-NBP2-19689-img0002.jpg "Western Blot: PAPD1 Antibody [NBP2-19689] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mt PAP antibody [N1C1] diluted at 1:3000.")
![Western Blot: PAPD1 Antibody [NBP2-19689] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Western-Blot-NBP2-19689-img0001.jpg)
![Western Blot: PAPD1 Antibody [NBP3-18648] - Figure 2. Western blot analysis using the antibody directed against PAPD1 Whole cell extracts of U2OS cells (80 ug) were analysed by Western blot using the antibody against PAPD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Western-Blot-NBP3-18648-img0001.jpg "Western Blot: PAPD1 Antibody [NBP3-18648] - Figure 2. Western blot analysis using the antibody directed against PAPD1 Whole cell extracts of U2OS cells (80 ug) were analysed by Western blot using the antibody against PAPD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left.")
![ELISA: PAPD1 Antibody [NBP3-18648] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PAPD1 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:47,000.](http://images.novusbio.com/fullsize/PAPD1 Antibody-ELISA-NBP3-18648-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PAPD1 Antibody [NBP2-55422] - Staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/PAPD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55422-img0001.jpg "Immunocytochemistry/Immunofluorescence: PAPD1 Antibody [NBP2-55422] - Staining of human cell line U-2 OS shows localization to mitochondria.")
![Immunohistochemistry-Paraffin: PAPD1 Antibody [NBP1-83856] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells).](http://images.novusbio.com/fullsize/PAPD1-Antibody-Immunohistochemistry-Paraffin-NBP1-83856-img0001.jpg "Immunohistochemistry-Paraffin: PAPD1 Antibody [NBP1-83856] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells).")