![Western Blot: PALB2 Antibody [NB100-60439] - Whole cell lysates from SUM159, MDA-MB-231, MCF-7 and HCC1954 cells were loaded with 50 ug/lane. 10% SDS-PAGE. PALB2 antibody (NB100-60439) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/PALB2-Antibody-Western-Blot-NB100-60439-img0007.jpg "Western Blot: PALB2 Antibody [NB100-60439] - Whole cell lysates from SUM159, MDA-MB-231, MCF-7 and HCC1954 cells were loaded with 50 ug/lane. 10% SDS-PAGE. PALB2 antibody (NB100-60439) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human partner and localizer of BRCA2 using the numbering given in entry NP_078951.2 (GeneID 79728). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PALB2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 11/15/2022 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for PALB2 Antibody (NB100-60439)Find related products by research area.
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The recent relationship of BRCA1 and 53BP1 The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage. DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m... Read full blog post. |
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![Western Blot: PALB2 Antibody [NB100-60439] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PALB2 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PALB2-Antibody-Western-Blot-NB100-60439-img0005.jpg "Western Blot: PALB2 Antibody [NB100-60439] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PALB2 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunoprecipitation: PALB2 Antibody [NB100-60439] - Detection of human PALB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PALB2 antibody NB100-60439 used for IP at 3 ug per reaction. PALB2 was also immunoprecipitated by rabbit anti-PALB2 antibody NB100-60440. For blotting immunoprecipitated PALB2, NB100-60439 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PALB2-Antibody-Immunoprecipitation-NB100-60439-img0006.jpg "Immunoprecipitation: PALB2 Antibody [NB100-60439] - Detection of human PALB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PALB2 antibody NB100-60439 used for IP at 3 ug per reaction. PALB2 was also immunoprecipitated by rabbit anti-PALB2 antibody NB100-60440. For blotting immunoprecipitated PALB2, NB100-60439 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
