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PAK6 Antibody

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Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat PAK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PAK6 at approximately 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 3.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A431 human epithelial carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4265-SP
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PAK6 Antibody Summary

Immunogen
E. coli-derived recombinant human PAK6
Met1-Arg137
Accession # Q9NQU5
Specificity
Detects endogenous human, mouse and rat PAK6 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAK6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF4265 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAK6 Antibody

  • EC 2.7.11
  • p21 protein (Cdc42/Rac)-activated kinase 6
  • p21(CDKN1A)-activated kinase 6
  • p21-activated kinase 6
  • p21-activated protein kinase 6
  • PAK-5
  • PAK5EC 2.7.11.1
  • PAK6
  • PAK-6
  • serine/threonine-protein kinase PAK 6

Background

As downstream targets of the Rho GTPases, the p21-activated kinase (PAK) family of serine/threonine kinases regulates the organization of the actin cytoskeleton in mammalian cells. The PAK family is structurally categorized in two groups, each with three members: group I PAK1-3; and group II PAK4-6. PAK6 is expressed most highly in brain and testes, with lower levels in multiple tissues. Both MKK6 and p38 MAPK activate PAK6, suggesting a role for this kinase in the cellular stress response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAK6 Antibody (AF4265)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(2)
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(2)
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Bioinformatics

Gene Symbol PAK6
Uniprot