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PKN2 Antibody (1D1) - BSA Free

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Western Blot: PKN2 Antibody (1D1) [NBP2-37573] - Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Western Blot: PKN2 Antibody (1D1) [NBP2-37573] - Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.
Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) [NBP2-37573] - Analysis of lung cancer tissues using PRK2 mouse mAb with DAB staining.
Flow Cytometry: PKN2 Antibody (1D1) [NBP2-37573] - Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).
Western Blot: PKN2 Antibody (1D1) [NBP2-37573] - Western blot analysis using PRK2 mAb against human PRK2 (AA: 555-718) recombinant protein. (Expected MW is 43.9 kDa)
Immunohistochemistry-Paraffin: PKN2 Antibody (1D1) [NBP2-37573] - Analysis of prostate tissues using PRK2 mouse mAb with DAB staining.
ELISA: PKN2 Antibody (1D1) [NBP2-37573] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
1D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37573-0.025ml
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PKN2 Antibody (1D1) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PKN2 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PKN2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for PKN2 Antibody (1D1) - BSA Free

  • cardiolipin-activated protein kinase Pak2
  • EC 2.7.11
  • EC 2.7.11.13
  • PAK2
  • Pak-2
  • PKN2
  • PRK2
  • PRK2MGC150606
  • PRKCL2
  • PRKCL2PRO2042
  • PRO2042
  • Protein kinase C-like 2MGC71074
  • protein kinase N2
  • Protein-kinase C-related kinase 2
  • serine/threonine-protein kinase N2

Background

Protein-kinase-C-related kinases (PRKs) (1) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN (2-3). Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PKN2