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PAF1 Antibody

Images

 
Western Blot: PAF1 Antibody [NBP2-19675] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry: PAF1 Antibody [NBP2-19675] - PAF1 antibody detects Paf1 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. PAF1 ...read more

Product Details

Summary
Reactivity Mu, Ze, Hu, Rt, Bv, Pm, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PAF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PAF1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Human (100%), Rat (100%), Primate (100%), Xenopus (93%), Bovine (100%), Zebrafish (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAF1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PAF1 Antibody

  • F23149_1
  • FLJ11123
  • Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
  • Pancreatic differentiation protein 2
  • PD2hPAF1
  • RNA polymerase II-associated factor 1 homolog

Background

PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PAF1 Antibody (NBP2-19675) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-19675

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Bioinformatics

Gene Symbol PAF1
Uniprot