Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (Hs-14) [NB500-455] - Intra Acrosomal Protein Antibody (Hs-14) [NB500-455] - Analysis of VCP in acetone-permeabilized human sperms using monoclonal antibody ...read more
Separation of human sperm cells stained using anti-VCP (Hs-14) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from human sperm cells unstained by primary antibody (GAM APC, black-dashed) in ...read more
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
VCP
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Staining technique: Membrane permeabilization (acetone) is essential. Application note: The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
Theoretical MW
220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB500-455 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
TBS (pH 8.0)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Multi-step
Alternate Names for p97/VCP Antibody (Hs-14)
15S Mg(2+)-ATPase p97 subunit
IBMPFD
IBMPFDMGC8560
MGC131997
p97
TER ATPase
TERA
transitional endoplasmic reticulum ATPase
valosin containing protein
Valosin-containing proteinMGC148092
VCP
yeast Cdc48p homolog
Background
This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our p97/VCP Antibody (Hs-14) and receive a gift card or discount.