![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Hela S3 NE.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0005.jpg "Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Hela S3 NE.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 4A8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | VCP (AAH07562, 1 a.a. ~ 588 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG |
| Specificity | VCP - valosin-containing protein (4A8) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | VCP |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publications using H00007415-M03 | Applications | Species |
|---|---|---|
| You X, Xu D, Zhang D et al. PYR-41 and Thalidomide Impair Dendritic Cell Cross-Presentation by Inhibiting Myddosome Formation and Attenuating the Endosomal Recruitments of p97 and Sec61 via NF-?B Inactivation. J Immunol Res. 2018-07-05 [PMID: 30069488] | ||
| Chang CP, Chan YY, Li CF et al. Deciphering the Molecular Mechanism Underlying the Inhibitory Efficacy of Taiwanese Local Pomegranate Peels against Urinary Bladder Urothelial Carcinoma. Nutrients 2018-04-27 [PMID: 29702555] | ||
| Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for p97/VCP Antibody (H00007415-M03)Find related products by research area.
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![Immunohistochemistry-Paraffin: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Immunohistochemistry-Paraffin-H00007415-M03-img0002.jpg "Immunohistochemistry-Paraffin: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml")
![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in HepG2 ( Cat # L019V1 ).](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0001.jpg "Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in HepG2 ( Cat # L019V1 ).")
![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in PC-12.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0004.jpg "Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in PC-12.")
![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Raw 264.7.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0006.jpg "Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Raw 264.7.")
![ELISA: p97/VCP Antibody (4A8) [H00007415-M03] - Detection limit for recombinant GST tagged VCP is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-ELISA-H00007415-M03-img0007.jpg "ELISA: p97/VCP Antibody (4A8) [H00007415-M03] - Detection limit for recombinant GST tagged VCP is 1 ng/ml as a capture antibody.")
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