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p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free

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Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S6K (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S6K.
Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S6K (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY S6K.
Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-S6K mouse monoclonal antibody.
Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more
Western Blot: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.
Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-S6K mouse monoclonal antibody.
Immunohistochemistry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Staining of paraffin-embedded Human pancreas tissue using anti-S6K mouse monoclonal antibody.
Flow Cytometry: p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free [NBP2-73209] - Analysis of Jurkat cells, using anti-S6K antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73209AF350
Alexa Fluor 405
NBP2-73209AF405
Alexa Fluor 488
NBP2-73209AF488
Alexa Fluor 532
NBP2-73209AF532
Alexa Fluor 594
NBP2-73209AF594
Alexa Fluor 647
NBP2-73209AF647
Alexa Fluor 700
NBP2-73209AF700
Alexa Fluor 750
NBP2-73209AF750
Allophycocyanin
NBP2-73209APC
Allophycocyanin/Cy7
NBP2-73209APCCY7
Biotin
NBP2-73209B
CoraFluor 1
NBP2-73209CL1
DyLight 350
NBP2-73209UV
DyLight 405
NBP2-73209V
DyLight 488
NBP2-73209G
DyLight 550
NBP2-73209R
DyLight 594
NBP2-73209DL594
DyLight 650
NBP2-73209C
DyLight 680
NBP2-73209FR
DyLight 755
NBP2-73209IR
FITC
NBP2-73209F
HRP
NBP2-73209H
Janelia Fluor 525
NBP2-73209JF525
Janelia Fluor 549
NBP2-73209JF549
Janelia Fluor 585
NBP2-73209JF585
Janelia Fluor 635
NBP2-73209JF635
Janelia Fluor 646
NBP2-73209JF646
Janelia Fluor 669
NBP2-73209JF669
PE
NBP2-73209PE
PE/Atto594
NBP2-73209PEATT594
PE/Cy5.5
NBP2-73209PECY55
PE/Cy7
NBP2-73209PECY7
PerCP
NBP2-73209PCP
Unconjugated
NBP2-00754
mFluor Violet 450 SE
NBP2-73209MFV450
mFluor Violet 500 SE
NBP2-73209MFV500
mFluor Violet 610 SE
NBP2-73209MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00754
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p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human RPS6KB1(NP_003152) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPS6KB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for p70 S6 Kinase/S6K Antibody (OTI1G4) - Azide and BSA Free

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

S6K encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RPS6KB1