![Immunohistochemistry: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32693) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-298202414262638.jpg "Immunohistochemistry: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32693) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | PSH03-58 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human p38 gamma/SAPK3 aa 1-367 / 367. (Uniprot: P53778) |
| Localization | Cytoplasm, Nucleus, Mitochondrion. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MAPK12 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for p38 gamma/SAPK3 Antibody (NBP3-32693)Find related products by research area.
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| Gene Symbol | MAPK12 |
![Immunocytochemistry/ Immunofluorescence: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Immunocytochemistry analysis of HeLa cells labeling p38 gamma/SAPK3 with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-2920241192310.jpg "Immunocytochemistry/ Immunofluorescence: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Immunocytochemistry analysis of HeLa cells labeling p38 gamma/SAPK3 with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Flow cytometric analysis of HeLa cells labeling p38 gamma/SAPK3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32693, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-29202416372126.jpg "Flow Cytometry: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Flow cytometric analysis of HeLa cells labeling p38 gamma/SAPK3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32693, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Western blot analysis of p38 gamma/SAPK3 on different lysates with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse heart tissue lysate Lane 7: Mouse skeletal muscle tissue lysate Lane 8: Rat skeletal muscle tissue lysate Lane 9: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32693_rabbit-p38-gamma-sapk3-mab-psh03-58-3920248485024.jpg "Western Blot: p38 gamma/SAPK3 Antibody (PSH03-58) [NBP3-32693] - Western blot analysis of p38 gamma/SAPK3 on different lysates with Rabbit anti-p38 gamma/SAPK3 antibody (NBP3-32693) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse heart tissue lysate Lane 7: Mouse skeletal muscle tissue lysate Lane 8: Rat skeletal muscle tissue lysate Lane 9: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
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