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OSTM1 Antibody

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Western Blot: OSTM1 Antibody [NBP2-19641] - Sample (30 ug of whole cell lysate) A: Raji B: HL-60 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Bv, Pm, RbSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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OSTM1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human OSTM1. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Predicted Species
Bovine (90%), Rabbit (92%), Monkey (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OSTM1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (81%), Rat (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for OSTM1 Antibody

  • GIPN
  • GLGAIP-interacting protein N terminus
  • grey-lethal osteopetrosis
  • HSPC019
  • OPTB5
  • osteopetrosis associated transmembrane protein 1
  • osteopetrosis-associated transmembrane protein 1

Background

This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for OSTM1 Antibody (NBP2-19641) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for OSTM1 Antibody (NBP2-19641). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in Ostm1 antibodies, but you are selling two antibodies against human protein; the first recognizing a band of 37 kDa (NBP2-19641) and the second recognizing a band between 55 and 72 kDa. What is the difference between these two bands? Please, could you tell me if with one of these two antibodies, we are able to detect mouse proteins?
    • For our OSTM1 antibodies, NBP2-19641 is detecting the endogenous protein in cell lysates Raji and HL-60. NBP1-81829 is detecting an overexpression lysate expressing a transfected DDK tagged OSTM1 protein. The DDK tag adds 26kDa to the molecular weight causing it to appear higher in the gel. We have not tested either of these products with mouse samples, so we are not certain if they will recognize the mouse protein. I have run an alignment of the full proteins for human and mouse. NBP1-81829 had an 85% homology to the mouse sequence and NBP2-19641 had an 81% homology. There is potential for these antibodies to recognize the mouse protein. If you decide you want to try one of these antibodies, you can take advantage of our Innovator's Reward program. In exchange of a review of our products in an untested application or species, we will issue you a credit for the purchase price of the antibody. Regardless of results.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OSTM1
Uniprot