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OSBPL9 Antibody

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Immunohistochemistry-Paraffin: OSBPL9 Antibody [NB100-1336] - (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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OSBPL9 Antibody Summary

Immunogen
Peptide with sequence C-EPLLKRLGAAKH corresponding to C-Terminus according to NP_078862.4, NP_683702.1, NP_683704.2, NP_683705.1, NP_683706.3, NP_683707.3.
Specificity
This antibody is expected to recognise all six human isoforms (NP_078862.4; NP_683702.1; NP_683704.2; NP_683705.1; NP_683706.3; NP_683707.3). Reported variants represent identical protein (NP_683702.1; NP_683703.1).
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OSBPL9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band and 19 kDa band in lysates of human brain, and testis and 55 kDa band in lysates of human placenta and testis after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the different calculated sizes of the six reported isoforms (a: 62.5 kDa band for NP_683702.1 and NP_683703.1; b: 63.9 kDa band for NP_683704.1; c: 70.2 kDa band for NP_683705.1; d: 81.8 kDa band for NP_683706.1; e: 83.2 kDa band for NP_078862.2; f: 84.3 kDa band for NP_683707.1). All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human Lung shows staining of alveolar macrophages..
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
OSBPL9 Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
OSBPL9 Peptide (NB100-1336PEP)
Publications
Read Publications using
NB100-1336 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OSBPL9 Antibody

  • FLJ12492
  • FLJ14629
  • FLJ14801
  • FLJ32055
  • FLJ34384
  • ORP-9
  • ORP9MGC15035
  • OSBP4
  • OSBP-related protein 9
  • oxysterol binding protein-like 9
  • oxysterol-binding protein-related protein 9

Background

The OSBPL9 gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors.Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-likesterol-binding domain, although so

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OSBPL9 Antibody (NB100-1336)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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WB Video Protocol

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Bioinformatics

Gene Symbol OSBPL9
Entrez
Uniprot