Western Blot: ORP1 Antibody [NB100-1331] - (0.5ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: ORP1 Antibody [NB100-1331] - Analysis of ORP1 in Human Muscle lysate using NB100-1331 at 0.5 ug/ ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot: Approx 48kDa band observed in Human Muscle lysate (predicted MW of 50kDa according to NP_060500). IHC: Paraffin embedded Human Brain (Cerebellum) and Human Skeletal Muscle.
Mouse reactivity reported in scientific literature (PMID: 22241858).
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified
Alternate Names for ORP1 Antibody
FLJ10217
ORP-1oxysterol-binding protein-related protein 1 variant 1
ORP1oxysterol-binding protein-related protein 1
OSBP8
OSBPL1
OSBPL1B
OSBP-related protein 1
oxysterol binding protein-like 1A
oxysterol binding protein-like 1B
oxysterol-binding protein-related protein 1 variant 2
Background
Oxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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