![Western Blot: Orexin Receptor 1/2 Antibody [NB100-93465] - Staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Orexin-Receptor-1-2-Antibody-Western-Blot-NB100-93465-img0003.jpg "Western Blot: Orexin Receptor 1/2 Antibody [NB100-93465] - Staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-YNFLSGKFREQFK corresponding to internal region according to NP_001517.2. |
| Specificity | This antibody is expected to recognise both the human proteins OX1R and OX2R. |
| Predicted Species | Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | HCRTR1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 48 kDa band observed in human brain (frontal cortex), mouse brain and rat brain lysates (calculated MW of 47.5 kDa band according to NP_001516.1 and NP_001517.1). |
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| Theoretical MW | 48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-93465 | Applications | Species |
|---|---|---|
| Fukunaka Y, Shinkai T, Hwang R et al. The orexin 1 receptor (HCRTR1) gene as a susceptibility gene contributing to polydipsia-hyponatremia in schizophrenia Neuromolecular Med 2007-01-01 [PMID: 17999203] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Orexin Receptor 1/2 Antibody (NB100-93465)Find related products by research area.
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