![Western Blot: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Western blot analysis using ONECUT3 mAb against HEK293 (1) and ONECUT3 (AA: 350-455)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ONECUT3-Antibody-10B2F5-Western-Blot-NBP2-37340-img0003.jpg "Western Blot: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Western blot analysis using ONECUT3 mAb against HEK293 (1) and ONECUT3 (AA: 350-455)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 10B2F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human ONECUT3 (AA: 350-455) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ONECUT3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Intracellular Staining by Flow Cytometry IL-17B Antibody [Unconjugated]](https://images.novusbio.com/images2/IL-17B_AF1709_Flow_Cytometry_8161.jpg)
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![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ONECUT3 |
![Immunohistochemistry-Paraffin: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Analysis of rectum cancer tissues using ONECUT3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ONECUT3-Antibody-10B2F5-Immunohistochemistry-NBP2-37340-img0005.jpg "Immunohistochemistry-Paraffin: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Analysis of rectum cancer tissues using ONECUT3 mouse mAb with DAB staining.")
![Western Blot: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Western blot analysis using ONECUT3 mAb against human ONECUT3 recombinant protein. (Expected MW is 38.2 kDa)](http://images.novusbio.com/fullsize/ONECUT3-Antibody-10B2F5-Western-Blot-NBP2-37340-img0002.jpg "Western Blot: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Western blot analysis using ONECUT3 mAb against human ONECUT3 recombinant protein. (Expected MW is 38.2 kDa)")
![Immunohistochemistry-Paraffin: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Analysis of cervical cancer tissues using ONECUT3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ONECUT3-Antibody-10B2F5-Immunohistochemistry-NBP2-37340-img0004.jpg "Immunohistochemistry-Paraffin: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Analysis of cervical cancer tissues using ONECUT3 mouse mAb with DAB staining.")
![ELISA: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ONECUT3-Antibody-10B2F5-ELISA-NBP2-37340-img0001.jpg "ELISA: ONECUT3 Antibody (10B2F5) [NBP2-37340] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![Immunocytochemistry HNF-6/ONECUT1 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af6277_human-hnf-6-onecut1-affinity-purified-polyclonal-ab-immunocytochemistry-3102023124257.jpg)
![Immunocytochemistry ONECUT2/OC-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ONECUT2_AF6294_Immunocytochemistry_10608.jpg)
![Immunocytochemistry ONECUT2/OC-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/af6294_human-onecut2-oc-2-affinity-purified-polyclonal-ab-immunocytochemistry-1832022103352.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")