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ONECUT2/OC-2 Antibody [Unconjugated]

 (17 Publications)
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ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room ...read more
ONECUT2/OC‑2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ONECUT2/OC‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6294-SP
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ONECUT2/OC-2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ONECUT2/OC-2
Gln185-Thr326
Accession # O95948
Specificity
Detects human ONECUT2/OC-2 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ONECUT2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF6294 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ONECUT2/OC-2 Antibody [Unconjugated]

  • one cut domain family member 2
  • HNF-6B
  • OC2
  • OC-2
  • one cut homeobox 2
  • ONECUT2

Background

ONECUT2 (one "cut" domain family member 2; also OC-2 and HNF6 beta ) is a monomeric 54 kDa (predicted) member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic neural crest plus hepatic, pancreatic, and intestinal tissue. In the adult, it is found in hepatocytes and small intestine epithelial cells. ONECUT2 is a transcriptional regulator that is known to impact the expression of Ngn3, OPN and Thbs4. This likely contributes to embryonic cell differentiation and migration. Human ONECUT2 is 504 amino acids (aa) in length. It contains two DNA binding regions, one classified as a CUT domain (aa 331‑410) and another as a homeobox domain (aa 427-481). There is one potential alternative start site at Met20. Over aa 185-326, human ONECUT2 shares 98% aa identity with mouse ONECUT2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ONECUT2/OC-2 Antibody (AF6294)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Immunoprecipitation.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(5)
IHC-Fr
(1)
IHC-P
(1)
Immunohistochemistry
(2)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(8)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF6294 Applications Species
Trevisan, AJ;Han, K;Chapman, P;Kulkarni, AS;Hinton, JM;Ramirez, C;Klein, I;Gatto, G;Gabitto, MI;Menon, V;Bikoff, JB; The transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output bioRxiv : the preprint server for biology 2024-09-19 [PMID: 39345580] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Mehta, K;Daghsni, M;Raeisossadati, R;Xu, Z;Davis, E;Naidich, A;Wang, B;Tao, S;Pi, S;Chen, W;Kostka, D;Liu, S;Gross, JM;Kuwajima, T;Aldiri, I; A cis-regulatory module underlies retinal ganglion cell genesis and axonogenesis Cell reports 2024-05-31 [PMID: 38823017] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
J Zhang, JM Roberts, F Chang, J Schwakopf, ML Vetter Jarid2 promotes temporal progression of retinal progenitors via repression of Foxp1 Cell Reports, 2023-03-14;42(3):112237. 2023-03-14 [PMID: 36924502] (IHC, Mouse) IHC Mouse
Romanov R, Tretiakov E, Kastriti M et al. Molecular design of hypothalamus development Nature 5/6/2020 [PMID: 32499648]
Lu Y, Shiau F, Yi W et al. Single-Cell Analysis of Human Retina Identifies Evolutionarily Conserved and Species-Specific Mechanisms Controlling Development Dev. Cell 5/18/2020 [PMID: 32386599]
A Sridhar, A Hoshino, CR Finkbeiner, A Chitsazan, L Dai, AK Haugan, KM Eschenbach, DL Jackson, C Trapnell, O Bermingham, I Glass, TA Reh Single-Cell Transcriptomic Comparison of Human Fetal Retina, hPSC-Derived Retinal Organoids, and Long-Term Retinal Cultures Cell Rep, 2020-02-04;30(5):1644-1659.e4. 2020-02-04 [PMID: 32023475] (IHC, Human) IHC Human
M Toch, A Harris, O Schakman, E Kondratska, JL Boulland, N Dauguet, S Debrulle, C Baudouin, M Hidalgo-Fi, A Gow, JC Glover, F Tissir, F Clotman Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits Sci Rep, 2020-01-22;10(1):996. 2020-01-22 [PMID: 31969659] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Gauhar Masgutova, Audrey Harris, Benvenuto Jacob, Lynn M. Corcoran, Frédéric Clotman Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord Frontiers in Molecular Neuroscience 2019-11-07 [PMID: 31787878]
R Wei, X Ren, H Kong, Z Lv, Y Chen, Y Tang, Y Wang, L Xiao, S Hacibekiro, C Liang, A Nagy, R Bremner, D Chen Rb1/Rbl1/Vhl loss induces mouse subretinal angiomatous proliferation and hemangioblastoma JCI Insight, 2019-11-14;0(0):. 2019-11-14 [PMID: 31613797] (IHC-Fr, Mouse) IHC-Fr Mouse
Audrey Harris, Gauhar Masgutova, Amandine Collin, Mathilde Toch, Maria Hidalgo-Figueroa, Benvenuto Jacob et al. Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord Frontiers in Cellular Neuroscience 2019-06-05 [PMID: 31231191]
Show All 17 Publications.

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Bioinformatics

Gene Symbol ONECUT2
Uniprot