![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Analysis in human stomach and skeletal muscle tissues using NBP2-52971 antibody. Corresponding OCLN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0011.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Analysis in human stomach and skeletal muscle tissues using NBP2-52971 antibody. Corresponding OCLN RNA-seq data are presented for the same tissues.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CL1555 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: DKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEED |
| Epitope | TSPVDDFRQPRYSSG |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | OCLN |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
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![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Occludin Antibody (NBP2-52971)Find related products by research area.
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| Gene Symbol | OCLN |
![Western Blot: Occludin Antibody (CL1555) [NBP2-52971] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Western-Blot-NBP2-52971-img0009.jpg "Western Blot: Occludin Antibody (CL1555) [NBP2-52971] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
![Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1555) [NBP2-52971] - staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunocytochemistry-Immunofluorescence-NBP2-52971-img0010.jpg "Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1555) [NBP2-52971] - staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human prostate cancer shows strong apical membrane positivity in tumor cells.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0016.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human prostate cancer shows strong apical membrane positivity in tumor cells.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human kidney shows membranous positivity in renal tubules.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0003.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human kidney shows membranous positivity in renal tubules.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human liver shows membranous immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0004.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human liver shows membranous immunoreactivity in hepatocytes.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human placenta shows membranous immunoreactivity in the trophoblast.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0005.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human placenta shows membranous immunoreactivity in the trophoblast.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human stomach shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0012.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human stomach shows strong positivity in apical membrane in glandular cells.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human colon shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0013.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human colon shows strong membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human fallopian tube shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0014.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human fallopian tube shows strong positivity in apical membrane in glandular cells.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human kidney shows moderate to strong positivity in apical membrane in cells in tubules.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0015.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human kidney shows moderate to strong positivity in apical membrane in cells in tubules.")
![Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Occludin-Antibody-CL1555-Immunohistochemistry-Paraffin-NBP2-52971-img0017.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (CL1555) [NBP2-52971] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
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![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
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